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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2761-2766. Prepublished online as a Blood First Edition Paper on July 8, 2004; DOI 10.1182/blood-2003-10-3614. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-10-3614v1 104/9/2761    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hristov, M. Articles by Weber, P. C. Search for Related Content PubMed PubMed Citation Articles by Hristov, M. Articles by Weber, P. C. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Apoptosis Signal Transduction Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Apoptotic bodies from endothelial cells enhance the number and initiate the differentiation of human endothelial progenitor cells in vitro Mihail Hristov, Wolfgang Erl, Stefan Linder, and Peter C. Weber From the Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Ludwig-Maximilians-Universität, München, Germany. Endothelial progenitor cells (EPCs) play a role in the repair of ischemic or injured tissue. Because endothelial injury can be associated with apoptosis, we have investigated whether apoptotic bodies from mature endothelial cells (ECs) may affect growth and differentiation of EPCs in vitro. A 24-hour incubation of isolated human EPCs with apoptotic bodies-rich medium (ABRM) from ECs led to a significant increase in the number of spindle-shaped attached cells. EPCs were characterized by DiI-Ac-LDL/lectin staining and measurement of CD34 and kinase insert domain receptor (KDR) expression. The treatment with ABRM resulted in a 2-fold increase of DiI-Ac-LDL/lectin-positive cells and up-regulation of CD34 (22% ± 2% versus 13% ± 3%, P < .05 and KDR (49% ± 12% versus 19% ± 7%, P < .05). Fluorescence and confocal laser microscopy demonstrated the uptake of apoptotic bodies by the EPCs. Apoptotic bodies-depleted medium had no effect, whereas the incubation with suspension of apoptotic bodies induced effects similar to those of ABRM. Our results suggest that apoptotic bodies from ECs are taken up by EPCs, increasing their number and differentiation state. Such a mechanism may facilitate the repair of injured endothelium and may represent a new signaling pathway between progenitor and damaged somatic cells. (Blood. 2004;104:2761-2766) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: The endothelial life insurance plan David C. Sane Blood 2004 104: 2615-2616. [Full Text] [PDF] This article has been cited by other articles: S. Nogueras, A. Merino, R. Ojeda, J. Carracedo, M. Rodriguez, A. Martin-Malo, R. Ramirez, and P. Aljama Coupling of endothelial injury and repair: an analysis using an in vivo experimental model Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H708 - H713. [Abstract] [Full Text] [PDF] S. Burghoff, Z. Ding, S. Godecke, A. Assmann, A. Wirrwar, D. Buchholz, O. Sergeeva, C. Leurs, H. Hanenberg, H.-W. Muller, et al. 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