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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2936-2939. Prepublished online as a Blood First Edition Paper on July 8, 2004; DOI 10.1182/blood-2004-01-0243. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-01-0243v1 104/9/2936    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shen, Y. Articles by Chan, W. C. Search for Related Content PubMed PubMed Citation Articles by Shen, Y. Articles by Chan, W. C. Related Collections Neoplasia Apoptosis Oncogenes and Tumor Suppressors Gene Expression Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Brief report BCL2 protein expression parallels its mRNA level in normal and malignant B cells Yulei Shen, Javeed Iqbal, James Z. Huang, Guimei Zhou, and Wing C. Chan From the Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE; and Department of Pathology, Oregon University Health & Science Center, Portland, OR. The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated posttranscriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Geyeregger, M. Shehata, M. Zeyda, F. W. Kiefer, K. M. Stuhlmeier, E. Porpaczy, G. J. Zlabinger, U. Jager, and T. M. Stulnig Liver X receptors interfere with cytokine-induced proliferation and cell survival in normal and leukemic lymphocytes J. Leukoc. Biol., November 1, 2009; 86(5): 1039 - 1048. [Abstract] [Full Text] [PDF] J. Iqbal, V. T. Neppalli, G. Wright, B. J. Dave, D. E. Horsman, A. Rosenwald, J. Lynch, C. P. Hans, D. D. Weisenburger, T. C. Greiner, et al. BCL2 Expression Is a Prognostic Marker for the Activated B-Cell-Like Type of Diffuse Large B-Cell Lymphoma J. Clin. Oncol., February 20, 2006; 24(6): 961 - 968. [Abstract] [Full Text] [PDF]

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