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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2981-2987. Prepublished online as a Blood First Edition Paper on July 15, 2004; DOI 10.1182/blood-2003-11-3961. This Article Full Text Full Text (PDF) Supplemental Table All Versions of this Article: 2003-11-3961v1 104/9/2981    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kolar, G. R. Articles by Capra, J. D. Search for Related Content PubMed PubMed Citation Articles by Kolar, G. R. Articles by Capra, J. D. Related Collections Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Human fetal, cord blood, and adult lymphocyte progenitors have similar potential for generating B cells with a diverse immunoglobulin repertoire Grant R. Kolar, Takafumi Yokota, Maria Isabel D. Rossi, Swapan K. Nath, and J. Donald Capra From the Program in Molecular Immunogenetics, the Program in Immunobiology and Cancer, and the Program in Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK; and the Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK. Several characteristics of the immunoglobulin (Ig) repertoire in fetuses and adults set them apart from each other. Functionally, this translates into differences in the affinity and effectiveness of the humoral immune response between adults and the very young. At least 2 possibilities could explain these differences: (1) fetal and adult lymphocyte progenitors differ significantly in their potential to form a diverse repertoire, and (2) factors extrinsic to the immunoglobulin locus are more influential to the character of the repertoire. To address this we used nonobese diabetic-severe combined immunodeficient-2 microglobulin knockout (NOD/SCID/2m-/-) mice reconstituted with human B-cell progenitors to compare the immunoglobulin repertoire potential of human fetal, cord blood, and adult sources. We found nearly identical VH and JH gene segment use and only modest differences in the third complementarity determining region of the immunoglobulin heavy chain (HCDR3). We conclude that the repertoire potential is remarkably similar regardless of the age of the individual from which progenitors are derived. Age-related differences in the immunoglobulin repertoire and variance of B-cell responses to immunization appear to arise from selection rather than from changes in recombination of the immunoglobulin locus itself. From the standpoint of the Ig repertoire, an immune system reconstituted from fetal or neonatal stem cells would likely be as diverse as one generated from adult bone marrow. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. R. Kolar, D. Mehta, R. Pelayo, and J. D. Capra A novel human B cell subpopulation representing the initial germinal center population to express AID Blood, March 15, 2007; 109(6): 2545 - 2552. [Abstract] [Full Text] [PDF] M. Zemlin, G. Hoersch, C. Zemlin, A. Pohl-Schickinger, M. Hummel, C. Berek, R. F. Maier, and K. Bauer The Postnatal Maturation of the Immunoglobulin Heavy Chain IgG Repertoire in Human Preterm Neonates Is Slower than in Term Neonates J. Immunol., January 15, 2007; 178(2): 1180 - 1188. [Abstract] [Full Text] [PDF]

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