Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 January 2005, Vol. 105, No. 1, pp. 282-288.
Prepublished online as a Blood First Edition Paper on September 2, 2004; DOI 10.1182/blood-2004-07-2782.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-07-2782v1
105/1/282    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mavropoulos, A.
Right arrow Articles by Clark, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mavropoulos, A.
Right arrow Articles by Clark, A. R.
Related Collections
Right arrow Immunobiology
Right arrow Signal Transduction
Right arrow Gene Expression
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

IMMUNOBIOLOGY

Stabilization of IFN-{gamma} mRNA by MAPK p38 in IL-12– and IL-18–stimulated human NK cells

Athanasios Mavropoulos, Gareth Sully, Andrew P. Cope, and Andrew R. Clark

From the Kennedy Institute of Rheumatology Division, Imperial College London, London, United Kingdom.

The rapid induction of interferon-{gamma} (IFN-{gamma}) by innate cytokines such as interleukin 12 (IL-12) and IL-18 is critical for immunity against infectious pathogens. We investigated the molecular mechanisms underlying this response. IL-12 and IL-18 rapidly and synergistically induced the secretion of IFN-{gamma} by freshly purified human peripheral blood lymphocytes. At early time points, IFN-{gamma} was expressed almost exclusively by natural killer cells and in both CD56bright and CD56dim subpopulations. Mitogen-activated protein kinase p38 was activated strongly by IL-18 and weakly by IL-12 in natural killer cells but was not activated by either cytokine in T cells. The expression of IFN-{gamma} mRNA and protein was dose-dependently blocked by SB203580, a specific inhibitor of mitogen-activated protein kinase p38, which also caused a dramatic destabilization of IFN-{gamma} mRNA. The 3' untranslated region (UTR) of IFN-{gamma} mRNA conferred p38 responsiveness to a heterologous reporter mRNA. Therefore, the synergistic induction of IFN-{gamma} by IL-12 and IL-18 in natural killer cells is mediated at least in part by p38-dependent and 3' UTR-mediated stabilization of IFN-{gamma} mRNA. (Blood. 2005;105:282-288)


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
V. Athie-Morales, G. M. O'Connor, and C. M. Gardiner
Activation of Human NK Cells by the Bacterial Pathogen-Associated Molecular Pattern Muramyl Dipeptide
J. Immunol., March 15, 2008; 180(6): 4082 - 4089.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Schierloh, N. Yokobori, M. Aleman, V. Landoni, L. Geffner, R. M. Musella, J. Castagnino, M. Baldini, E. Abbate, S. S. de la Barrera, et al.
Mycobacterium tuberculosis-Induced Gamma Interferon Production by Natural Killer Cells Requires Cross Talk with Antigen-Presenting Cells Involving Toll-Like Receptors 2 and 4 and the Mannose Receptor in Tuberculous Pleurisy
Infect. Immun., November 1, 2007; 75(11): 5325 - 5337.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. M. Abraham, T. Lawrence, A. Kleiman, P. Warden, M. Medghalchi, J. Tuckermann, J. Saklatvala, and A. R. Clark
Antiinflammatory effects of dexamethasone are partly dependent on induction of dual specificity phosphatase 1
J. Exp. Med., August 7, 2006; 203(8): 1883 - 1889.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. S. Berenson, J. Yang, B. P. Sleckman, T. L. Murphy, and K. M. Murphy
Selective Requirement of p38{alpha} MAPK in Cytokine-Dependent, but Not Antigen Receptor-Dependent, Th1 Responses.
J. Immunol., April 15, 2006; 176(8): 4616 - 4621.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. P. Luyendyk, L. D. Lehman-McKeeman, D. M. Nelson, V. M. Bhaskaran, T. P. Reilly, B. D. Car, G. H. Cantor, J. F. Maddox, P. E. Ganey, and R. A. Roth
Unique Gene Expression and Hepatocellular Injury in the Lipopolysaccharide-Ranitidine Drug Idiosyncrasy Rat Model: Comparison with Famotidine
Toxicol. Sci., April 1, 2006; 90(2): 569 - 585.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J.-S. Chang, J. F. Huggett, K. Dheda, L. U. Kim, A. Zumla, and G. A. W. Rook
Myobacterium tuberculosis Induces Selective Up-Regulation of TLRs in the Mononuclear Leukocytes of Patients with Active Pulmonary Tuberculosis.
J. Immunol., March 1, 2006; 176(5): 3010 - 3018.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020