SEARCH:   Advanced

Blood, 15 May 2005, Vol. 105, No. 10, pp. 3879-3887. Prepublished online as a Blood First Edition Paper on January 25, 2005; DOI 10.1182/blood-2004-04-1392. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-04-1392v1 105/10/3879    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Flaumenhaft, R. Articles by Dvorak, A. M. Search for Related Content PubMed PubMed Citation Articles by Flaumenhaft, R. Articles by Dvorak, A. M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cytoskeleton Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY The actin cytoskeleton differentially regulates platelet -granule and dense-granule secretion Robert Flaumenhaft, James R. Dilks, Nataliya Rozenvayn, Rita A. Monahan-Earley, Dian Feng, and Ann M. Dvorak From the Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA; and the Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA. Stimulation of platelets with strong agonists results in centralization of cytoplasmic organelles and secretion of granules. These observations have led to the supposition that cytoskeletal contraction facilitates granule release by promoting the interaction of granules with one another and with membranes of the open canalicular system. Yet, the influence of the actin cytoskeleton in controlling the membrane fusion events that mediate granule secretion remains largely unknown. To evaluate the role of the actin cytoskeleton in platelet granule secretion, we have assessed the effects of latrunculin A and cytochalasin E on granule secretion. Exposure of platelets to low concentrations of these reagents resulted in acceleration and augmentation of agonist-induced -granule secretion with comparatively modest effects on dense granule secretion. In contrast, exposure of platelets to high concentrations of latrunculin A inhibited agonist-induced -granule secretion but stimulated dense granule secretion. Incubation of permeabilized platelets with low concentrations of latrunculin A primed platelets for Ca2+- or guanosine triphosphate (GTP)--S-induced -granule secretion. Latrunculin A-dependent -granule secretion was inhibited by antibodies directed at vesicle-associated membrane protein (VAMP), demonstrating that latrunculin A supports soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein-dependent membrane fusion. These results indicate that the actin cytoskeleton interferes with platelet exocytosis and differentially regulates -granule and dense granule secretion. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. I. Fortoul, A. Gonzalez-Villalva, G. Pinon-Zarate, V. Rodriguez-Lara, L. F. Montano, and L. Saldivar-Osorio Ultrastructural megakaryocyte modifications after vanadium inhalation in spleen and bone marrow J. Electron Microsc. (Tokyo), December 1, 2009; 58(6): 375 - 380. [Abstract] [Full Text] [PDF] L. Lian, Y. Wang, M. Flick, J. Choi, E. W. Scott, J. Degen, M. A. Lemmon, and C. S. Abrams Loss of pleckstrin defines a novel pathway for PKC-mediated exocytosis Blood, April 9, 2009; 113(15): 3577 - 3584. [Abstract] [Full Text] [PDF] K. L. Tucker, T. Sage, J. M. Stevens, P. A. Jordan, S. Jones, N. E. Barrett, R. St-Arnaud, J. Frampton, S. Dedhar, and J. M. Gibbins A dual role for integrin-linked kinase in platelets: regulating integrin function and {alpha}-granule secretion Blood, December 1, 2008; 112(12): 4523 - 4531. [Abstract] [Full Text] [PDF] R. Flaumenhaft, N. Rozenvayn, D. Feng, and A. M. Dvorak SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane Blood, September 1, 2007; 110(5): 1492 - 1501. [Abstract] [Full Text] [PDF] D. S. Sim, J. R. Dilks, and R. Flaumenhaft Platelets Possess and Require an Active Protein Palmitoylation Pathway for Agonist-Mediated Activation and In Vivo Thrombus Formation Arterioscler Thromb Vasc Biol, June 1, 2007; 27(6): 1478 - 1485. [Abstract] [Full Text] [PDF] N. R. Jog, M. J. Rane, G. Lominadze, G. C. Luerman, R. A. Ward, and K. R. McLeish The actin cytoskeleton regulates exocytosis of all neutrophil granule subsets Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1690 - C1700. [Abstract] [Full Text] [PDF] J.-S. Huang, L. Dong, T. Kozasa, and G. C. Le Breton Signaling through G{alpha}13 Switch Region I Is Essential for Protease-activated Receptor 1-mediated Human Platelet Shape Change, Aggregation, and Secretion J. Biol. Chem., April 6, 2007; 282(14): 10210 - 10222. [Abstract] [Full Text] [PDF] N. R. Jog, V. R. Jala, R. A. Ward, M. J. Rane, B. Haribabu, and K. R. McLeish Heat Shock Protein 27 Regulates Neutrophil Chemotaxis and Exocytosis through Two Independent Mechanisms J. Immunol., February 15, 2007; 178(4): 2421 - 2428. [Abstract] [Full Text] [PDF] Q. Ren, H. K. Barber, G. L. Crawford, Z. A. Karim, C. Zhao, W. Choi, C.-C. Wang, W. Hong, and S. W. Whiteheart Endobrevin/VAMP-8 Is the Primary v-SNARE for the Platelet Release Reaction Mol. Biol. Cell, January 1, 2007; 18(1): 24 - 33. [Abstract] [Full Text] [PDF] Y. Wang, E. Vachon, J. Zhang, V. Cherepanov, J. Kruger, J. Li, K. Saito, P. Shannon, N. Bottini, H. Huynh, et al. Tyrosine phosphatase MEG2 modulates murine development and platelet and lymphocyte activation through secretory vesicle function J. Exp. Med., December 5, 2005; 202(11): 1587 - 1597. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020