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 Blood, 1 June 2005, Vol. 105, No. 11, pp. 4282-4289.
Prepublished online as a Blood First Edition Paper on February 17, 2005; DOI 10.1182/blood-2004-02-0756.

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HEMATOPOIESIS

Identification and characterization of undifferentiated mast cells in mouse bone marrow

Maria Célia Jamur, Ana Cristina G. Grodzki, Elsa H. Berenstein, Majed M. Hamawy, Reuben P. Siraganian, and Constance Oliver

From the Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto, Brasil; and the Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.

Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34+, CD13+, and c-kit+, and does not express Fc{epsilon}RI. However, by polymerase chain reaction (PCR) the cell contains message for the {alpha} and {beta} subunits of Fc{epsilon}RI, mast cell–specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell–specific gangliosides, Fc{epsilon}RI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.


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