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Blood, 1 June 2005, Vol. 105, No. 11, pp. 4314-4320. Prepublished online as a Blood First Edition Paper on February 8, 2005; DOI 10.1182/blood-2004-11-4418. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-11-4418v1 105/11/4314    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, C. C. Articles by Lodish, H. F. Search for Related Content PubMed PubMed Citation Articles by Zhang, C. C. Articles by Lodish, H. F. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion Cheng Cheng Zhang, and Harvey F. Lodish From the Whitehead Institute for Biomedical Research, Cambridge, MA, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, MA. Ex vivo expansion of hematopoietic stem cells (HSCs) is important for many clinical applications, and knowledge of the surface phenotype of ex vivo–expanded HSCs will be critical to their purification and analysis. Here, we developed a simple culture system for bone marrow (BM) HSCs using low levels of stem cell factor (SCF), thrombopoietin (TPO), insulin-like growth factor 2 (IGF-2), and fibroblast growth factor-1 (FGF-1) in serum-free medium. As measured by competitive repopulation analyses, there was a more than 20-fold increase in numbers of long-term (LT)–HSCs after a 10-day culture of total BM cells. Culture of BM "side population" (SP) cells, a highly enriched stem cell population, for 10 days resulted in an approximate 8-fold expansion of repopulating HSCs. Similar to freshly isolated HSCs, repopulating HSCs after culture were positive for the stem cell markers Sca-1, Kit, and CD31 and receptors for IGF-2. Surprisingly, prion protein and Tie-2, which are present on freshly isolated HSCs, were not on cultured HSCs. Two other HSC markers, Endoglin and Mpl, were expressed only on a portion of cultured HSCs. Therefore, the surface phenotype of ex vivo–expanded HSCs is different from that of freshly isolated HSCs, but this plasticity of surface phenotype does not significantly alter their repopulation capability. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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