SEARCH:   Advanced

Blood, 1 June 2005, Vol. 105, No. 11, pp. 4314-4320. Prepublished online as a Blood First Edition Paper on February 8, 2005; DOI 10.1182/blood-2004-11-4418. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-11-4418v1 105/11/4314    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, C. C. Articles by Lodish, H. F. Search for Related Content PubMed PubMed Citation Articles by Zhang, C. C. Articles by Lodish, H. F. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion Cheng Cheng Zhang, and Harvey F. Lodish From the Whitehead Institute for Biomedical Research, Cambridge, MA, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, MA. Ex vivo expansion of hematopoietic stem cells (HSCs) is important for many clinical applications, and knowledge of the surface phenotype of ex vivo–expanded HSCs will be critical to their purification and analysis. Here, we developed a simple culture system for bone marrow (BM) HSCs using low levels of stem cell factor (SCF), thrombopoietin (TPO), insulin-like growth factor 2 (IGF-2), and fibroblast growth factor-1 (FGF-1) in serum-free medium. As measured by competitive repopulation analyses, there was a more than 20-fold increase in numbers of long-term (LT)–HSCs after a 10-day culture of total BM cells. Culture of BM "side population" (SP) cells, a highly enriched stem cell population, for 10 days resulted in an approximate 8-fold expansion of repopulating HSCs. Similar to freshly isolated HSCs, repopulating HSCs after culture were positive for the stem cell markers Sca-1, Kit, and CD31 and receptors for IGF-2. Surprisingly, prion protein and Tie-2, which are present on freshly isolated HSCs, were not on cultured HSCs. Two other HSC markers, Endoglin and Mpl, were expressed only on a portion of cultured HSCs. Therefore, the surface phenotype of ex vivo–expanded HSCs is different from that of freshly isolated HSCs, but this plasticity of surface phenotype does not significantly alter their repopulation capability. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Huynh, S. Iizuka, M. Kaba, O. Kirak, J. Zheng, H. F. Lodish, and C. C. Zhang Insulin-Like Growth Factor-Binding Protein 2 Secreted by a Tumorigenic Cell Line Supports Ex Vivo Expansion of Mouse Hematopoietic Stem Cells Stem Cells, June 1, 2008; 26(6): 1628 - 1635. [Abstract] [Full Text] [PDF] H. Abdel-Azim, Y. Zhu, R. Hollis, X. Wang, S. Ge, Q.-L. Hao, G. Smbatyan, D. B. Kohn, M. Rosol, and G. M. Crooks Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl Blood, April 15, 2008; 111(8): 4064 - 4074. [Abstract] [Full Text] [PDF] Y. Amsalem, M. S. Feinberg, N. Landa, L. Miller, R. Holbova, O. Shaharabani-Yosef, I. M. Barbash, J. Leor, Y. Mardor, D. Daniels, et al. Response to Letter Regarding Article, "Iron-Oxide Labeling and Outcome of Transplanted Mesenchymal Stem Cells in the Infarcted Myocardium" Circulation, April 8, 2008; 117(14): e307 - e307. [Full Text] [PDF] C. C. Zhang, M. Kaba, S. Iizuka, H. Huynh, and H. F. Lodish Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation Blood, April 1, 2008; 111(7): 3415 - 3423. [Abstract] [Full Text] [PDF] S. Noda, K. Horiguchi, H. Ichikawa, and H. Miyoshi Repopulating Activity of Ex Vivo-Expanded Murine Hematopoietic Stem Cells Resides in the CD48-c-Kit+Sca-1+Lineage Marker- Cell Population Stem Cells, March 1, 2008; 26(3): 646 - 655. [Abstract] [Full Text] [PDF] U. Blank, G. Karlsson, and S. Karlsson Signaling pathways governing stem-cell fate Blood, January 15, 2008; 111(2): 492 - 503. [Abstract] [Full Text] [PDF] K. Aoyama, C. Delaney, B. Varnum-Finney, A. D. Kohn, R. T. Moon, and I. D. Bernstein The Interaction of the Wnt and Notch Pathways Modulates Natural Killer Versus T Cell Differentiation Stem Cells, October 1, 2007; 25(10): 2488 - 2497. [Abstract] [Full Text] [PDF] Y. Amsalem, Y. Mardor, M. S. Feinberg, N. Landa, L. Miller, D. Daniels, A. Ocherashvilli, R. Holbova, O. Yosef, I. M. Barbash, et al. Iron-Oxide Labeling and Outcome of Transplanted Mesenchymal Stem Cells in the Infarcted Myocardium Circulation, September 11, 2007; 116(11_suppl): I-38 - I-45. [Abstract] [Full Text] [PDF] M.-J. Liao, C. C. Zhang, B. Zhou, D. B. Zimonjic, S. A. Mani, M. Kaba, A. Gifford, F. Reinhardt, N. C. Popescu, W. Guo, et al. Enrichment of a Population of Mammary Gland Cells that Form Mammospheres and Have In vivo Repopulating Activity Cancer Res., September 1, 2007; 67(17): 8131 - 8138. [Abstract] [Full Text] [PDF] C. Peng, J. Brain, Y. Hu, A. Goodrich, L. Kong, D. Grayzel, R. Pak, M. Read, and S. Li Inhibition of heat shock protein 90 prolongs survival of mice with BCR-ABL-T315I-induced leukemia and suppresses leukemic stem cells Blood, July 15, 2007; 110(2): 678 - 685. [Abstract] [Full Text] [PDF] Y.-J. Kang, S.-J. Yang, G. Park, B. Cho, C.-K. Min, T.-Y. Kim, J.-S. Lee, and I.-H. Oh A Novel Function of Interleukin-10 Promoting Self-Renewal of Hematopoietic Stem Cells Stem Cells, July 1, 2007; 25(7): 1814 - 1822. [Abstract] [Full Text] [PDF] H. K. A. Mikkola and S. H. Orkin The journey of developing hematopoietic stem cells. Development, October 1, 2006; 133(19): 3733 - 3744. [Abstract] [Full Text] [PDF] N. Buza-Vidas, J. Antonchuk, H. Qian, R. Mansson, S. Luc, S. Zandi, K. Anderson, S. Takaki, J. M. Nygren, C. T. Jensen, et al. Cytokines regulate postnatal hematopoietic stem cell expansion: opposing roles of thrombopoietin and LNK Genes & Dev., August 1, 2006; 20(15): 2018 - 2023. [Abstract] [Full Text] [PDF] J. S.G. Yeoh, R. van Os, E. Weersing, A. Ausema, B. Dontje, E. Vellenga, and G. de Haan Fibroblast Growth Factor-1 and -2 Preserve Long-Term Repopulating Ability of Hematopoietic Stem Cells in Serum-Free Cultures Stem Cells, June 1, 2006; 24(6): 1564 - 1572. [Abstract] [Full Text] [PDF] B. Dykstra, J. Ramunas, D. Kent, L. McCaffrey, E. Szumsky, L. Kelly, K. Farn, A. Blaylock, C. Eaves, and E. Jervis High-resolution video monitoring of hematopoietic stem cells cultured in single-cell arrays identifies new features of self-renewal PNAS, May 23, 2006; 103(21): 8185 - 8190. [Abstract] [Full Text] [PDF] G. Larderet, N. O. Fortunel, P. Vaigot, M. Cegalerba, P. Maltere, O. Zobiri, X. Gidrol, G. Waksman, and M. T. Martin Human Side Population Keratinocytes Exhibit Long-Term Proliferative Potential and a Specific Gene Expression Profile and Can Form a Pluristratified Epidermis Stem Cells, April 1, 2006; 24(4): 965 - 974. [Abstract] [Full Text] [PDF] C. C. Zhang, A. D. Steele, S. Lindquist, and H. F. Lodish Prion protein is expressed on long-term repopulating hematopoietic stem cells and is important for their self-renewal PNAS, February 14, 2006; 103(7): 2184 - 2189. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020