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Blood, 15 June 2005, Vol. 105, No. 12, pp. 4657-4663. Prepublished online as a Blood First Edition Paper on March 3, 2005; DOI 10.1182/blood-2004-09-3554. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3554v1 105/12/4657    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hisatsune, H. Articles by Nishikawa, S.-I. Search for Related Content PubMed PubMed Citation Articles by Hisatsune, H. Articles by Nishikawa, S.-I. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY High level of endothelial cell-specific gene expression by a combination of the 5' flanking region and the 5' half of the first intron of the VE-cadherin gene Hiroshi Hisatsune, Kazuyoshi Matsumura, Minetaro Ogawa, Akiyoshi Uemura, Nobuyuki Kondo, Jun K. Yamashita, Hideto Katsuta, Satomi Nishikawa, Tsutomu Chiba, and Shin-Ichi Nishikawa From the Division of Gastroenterology and Hepatology, the Department of Internal Medicine, Graduate School of Medicine, the Department of Thoracic Surgery, the Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, and the Department of Ophthalmology and Visual Sciences, Kyoto University; the Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University; and the Stem Cell Biology Laboratory, Center for Developmental Biology, Kobe, Japan. To develop a tool to obtain a high level of gene expression specifically in endothelial cells (ECs), we assessed enhancer activity of fragments in the first intron of the VE-cadherin gene using 3 different experimental systems: luciferase assay in the F2 EC line, green fluorescent protein (GFP) expression in ECs generated in embryonic stem (ES) cell differentiation culture, and GFP expression in transgenic mice. Although the 2.5-kbp (kilobase pair) 5' flanking sequence of the VE-cadherin gene is EC specific, adding 4 kbp of the 5' half of the first intron affected an enhancement of the gene expression level in all 3 assay systems. No other fragments tested in this study could confer such effects. Compared with other gene expression units, the unit described in this study would be the most optimum one available to date for EC-specific gene expression. Because this unit can express genes in VE-cadherin+ progenitors of hematopoietic cells but not in fully committed hematopoietic cells, it will be useful to manipulate specifically the uncommitted progenitor stage during hematopoietic cell differentiation. (Blood. 2005; 105:4657-4663) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Vestweber VE-Cadherin: The Major Endothelial Adhesion Molecule Controlling Cellular Junctions and Blood Vessel Formation Arterioscler Thromb Vasc Biol, February 1, 2008; 28(2): 223 - 232. [Abstract] [Full Text] [PDF] Y. N. Tallini, J. F. Brekke, B. Shui, R. Doran, S.-m. Hwang, J. Nakai, G. Salama, S. S. Segal, and M. I. Kotlikoff Propagated Endothelial Ca2+ Waves and Arteriolar Dilation In Vivo: Measurements in Cx40BAC GCaMP2 Transgenic Mice Circ. Res., December 7, 2007; 101(12): 1300 - 1309. [Abstract] [Full Text] [PDF] Z. Li, J. C. Wu, A. Y. Sheikh, D. Kraft, F. Cao, X. Xie, M. Patel, S. S. Gambhir, R. C. Robbins, J. P. Cooke, et al. Differentiation, Survival, and Function of Embryonic Stem Cell Derived Endothelial Cells for Ischemic Heart Disease Circulation, September 11, 2007; 116(11_suppl): I-46 - I-54. [Abstract] [Full Text] [PDF] Z. Li, M. J. Chen, T. Stacy, and N. A. Speck Runx1 function in hematopoiesis is required in cells that express Tek Blood, January 1, 2006; 107(1): 106 - 110. [Abstract] [Full Text] [PDF]

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