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Blood, 15 June 2005, Vol. 105, No. 12, pp. 4674-4676. Prepublished online as a Blood First Edition Paper on February 24, 2005; DOI 10.1182/blood-2004-12-4701. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-12-4701v1 105/12/4674    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yarovoi, H. Articles by Poncz, M. Search for Related Content PubMed PubMed Citation Articles by Yarovoi, H. Articles by Poncz, M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Brief report Intracellular interaction of von Willebrand factor and factor VIII depends on cellular context: lessons from platelet-expressed factor VIII Helen Yarovoi, Alan T. Nurden, Robert R. Montgomery, Paquita Nurden, and Mortimer Poncz From the Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA; Institut Fédératif de Recherche No 4, Hôpital Cardiologique, Pessac, France; Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI; and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA. We have previously reported that ectopically expressed factor VIII (FVIII) is stored within platelets and is released upon platelet activation. Studies by others in various cell lines have suggested that having von Willebrand factor (VWF) coexpression is necessary for FVIII granular storage and for its secretion. We tested the importance of VWF coexpression for ectopic storage of FVIII in platelets and for its bioavailability. Transgenic mice expressing platelet-specific FVIII were crossed onto a VWF-/- background. Antigenic levels of platelet FVIII in these mice were nearly unchanged whether VWF was present or not. Whole-blood clotting times and FeCl3 carotid artery injury correction demonstrated that platelet FVIII demonstrably improved the bleeding diathesis in FVIIInull mice independent of the platelets' VWF status. Immunogold electron microscopy demonstrated that platelet FVIII is stored in platelet -granules independent of the presence of VWF. It appears that FVIII's interaction with VWF and its intracellular transportation, storage, and secretion differ greatly depending on the cell type. The molecular basis for these differences now needs to be elucidated. (Blood. 2005;105:4674-4676) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. L. Haberichter VWF and FVIII: the origins of a great friendship Blood, March 26, 2009; 113(13): 2873 - 2874. [Full Text] [PDF] M. Neyman, J. Gewirtz, and M. Poncz Analysis of the spatial and temporal characteristics of platelet-delivered factor VIII-based clots Blood, August 15, 2008; 112(4): 1101 - 1108. [Abstract] [Full Text] [PDF]

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