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Blood, 15 January 2005, Vol. 105, No. 2, pp. 567-575. Prepublished online as a Blood First Edition Paper on September 14, 2004; DOI 10.1182/blood-2004-03-1026. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-03-1026v1 105/2/567    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hidalgo, A. Articles by Frenette, P. S. Search for Related Content PubMed PubMed Citation Articles by Hidalgo, A. Articles by Frenette, P. S. Related Collections Cell Adhesion and Motility Hematopoiesis Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Enforced fucosylation of neonatal CD34+ cells generates selectin ligands that enhance the initial interactions with microvessels but not homing to bone marrow Andrés Hidalgo, and Paul S. Frenette From the Department of Medicine and Immunobiology Center, Mount Sinai School of Medicine, New York, NY. Hematopoietic progenitor/stem cell homing to the bone marrow requires the concerted action of several adhesion molecules. Endothelial P- and E-selectins play an important role in this process, but their ligands on a large subset of neonate-derived human CD34+ cells are absent, leading to a reduced ability to interact with the bone marrow (BM) microvasculature. We report here that this deficiency results from reduced 1,3-fucosyltransferase (FucT) expression and activity in these CD34+ cells. Incubation of CD34+ cells with recombinant human FucTVI rapidly corrected the deficiency in nonbinding CD34+ cells and further increased the density of ligands for both P- and E-selectins on all cord blood–derived CD34+ cells. Intravital microscopy studies revealed that these FucTVI-treated CD34+ cells displayed a marked enhancement in their initial interactions with the BM microvasculature, but unexpectedly, homing into the BM was not improved by FucTVI treatment. These data indicate that, although exogenous FucT enzyme activity can rapidly modulate selectin binding avidity of cord blood CD34+ cells, further studies are needed to understand how to translate a positive effect on progenitor cell adhesion in bone marrow microvessels into one that significantly influences migration and lodgement into the parenchyma. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. MENDEZ-FERRER and P. S. FRENETTE Hematopoietic Stem Cell Trafficking: Regulated Adhesion and Attraction to Bone Marrow Microenvironment Ann. N.Y. Acad. Sci., November 1, 2007; 1116(1): 392 - 413. [Abstract] [Full Text] [PDF] J.J. Mao, W.V. Giannobile, J.A. Helms, S.J. Hollister, P.H. Krebsbach, M.T. Longaker, and S. Shi Craniofacial tissue engineering by stem cells. J. Dent. Res., November 1, 2006; 85(11): 966 - 979. [Abstract] [Full Text] [PDF]

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