|
|
Blood, 15 January 2005, Vol. 105, No. 2, pp. 617-626.
Prepublished online as a Blood First Edition Paper on September 16, 2004; DOI 10.1182/blood-2004-04-1649.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
Human embryonic stem cell–derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential
Maxim A. Vodyanik,
Jack A. Bork,
James A. Thomson, and
Igor I. Slukvin
From the Wisconsin National Primate Research Center; the Department of Anatomy; the Department of Obstetrics and Gynecology; and the Department of Pathology and Laboratory Medicine, University of Wisconsin; and the WiCell Research Institute, Madison, WI.
Embryonic stem (ES) cells have the potential to serve as an alternative source of hematopoietic precursors for transplantation and for the study of hematopoietic cell development. Using coculture of human ES (hES) cells with OP9 bone marrow stromal cells, we were able to obtain up to 20% of CD34+ cells and isolate up to 107 CD34+ cells with more than 95% purity from a similar number of initially plated hES cells after 8 to 9 days of culture. The hES cell–derived CD34+ cells were highly enriched in colony-forming cells, cells expressing hematopoiesis-associated genes GATA-1, GATA-2, SCL/TAL1, and Flk-1, and retained clonogenic potential after in vitro expansion. CD34+ cells displayed the phenotype of primitive hematopoietic progenitors as defined by co-expression of CD90, CD117, and CD164, along with a lack of CD38 expression and contained aldehyde dehydrogenase–positive cells as well as cells with verapamil-sensitive ability to efflux rhodamine 123. When cultured on MS-5 stromal cells in the presence of stem cell factor, Flt3-L, interleukin 7 (IL-7), and IL-3, isolated CD34+ cells differentiated into lymphoid (B and natural killer cells) as well as myeloid (macrophages and granulocytes) lineages. These data indicate that CD34+ cells generated through hES/OP9 coculture display several features of definitive hematopoietic stem cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. B. Nourse, D. E. Halpin, M. Scatena, D. J. Mortisen, N. L. Tulloch, K. D. Hauch, B. Torok-Storb, B. D. Ratner, L. Pabon, and C. E. Murry
VEGF Induces Differentiation of Functional Endothelium From Human Embryonic Stem Cells: Implications for Tissue Engineering
Arterioscler Thromb Vasc Biol,
January 1, 2010;
30(1):
80 - 89.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Kaufman
Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells
Blood,
October 22, 2009;
114(17):
3513 - 3523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. McKinney-Freeman, O. Naveiras, F. Yates, S. Loewer, M. Philitas, M. Curran, P. J. Park, and G. Q. Daley
Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells
Blood,
July 9, 2009;
114(2):
268 - 278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yokoyama, T. Suzuki, M. Sakata-Yanagimoto, K. Kumano, K. Higashi, T. Takato, M. Kurokawa, S. Ogawa, and S. Chiba
Derivation of functional mature neutrophils from human embryonic stem cells
Blood,
June 25, 2009;
113(26):
6584 - 6592.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. S. Woll, B. Grzywacz, X. Tian, R. K. Marcus, D. A. Knorr, M. R. Verneris, and D. S. Kaufman
Human embryonic stem cells differentiate into a homogeneous population of natural killer cells with potent in vivo antitumor activity
Blood,
June 11, 2009;
113(24):
6094 - 6101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Timmermans, I. Velghe, L. Vanwalleghem, M. De Smedt, S. Van Coppernolle, T. Taghon, H. D. Moore, G. Leclercq, A. W. Langerak, T. Kerre, et al.
Generation of T Cells from Human Embryonic Stem Cell-Derived Hematopoietic Zones
J. Immunol.,
June 1, 2009;
182(11):
6879 - 6888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Kelly and K. K. Hirschi
Signaling Hierarchy Regulating Human Endothelial Cell Development
Arterioscler Thromb Vasc Biol,
May 1, 2009;
29(5):
718 - 724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Enzmann, E. Yolcu, H. J. Kaplan, and S. T. Ildstad
Stem Cells as Tools in Regenerative Therapy for Retinal Degeneration
Arch Ophthalmol,
April 1, 2009;
127(4):
563 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-J. Lu, Q. Feng, J. S. Park, L. Vida, B.-S. Lee, M. Strausbauch, P. J. Wettstein, G. R. Honig, and R. Lanza
Biologic properties and enucleation of red blood cells from human embryonic stem cells
Blood,
December 1, 2008;
112(12):
4475 - 4484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. T. Zambidis, T. Soon Park, W. Yu, A. Tam, M. Levine, X. Yuan, M. Pryzhkova, and B. Peault
Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells
Blood,
November 1, 2008;
112(9):
3601 - 3614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Su, C. Frye, K.-M. Bae, V. Kelley, and J. Vieweg
Differentiation of Human Embryonic Stem Cells into Immunostimulatory Dendritic Cells under Feeder-Free Culture Conditions
Clin. Cancer Res.,
October 1, 2008;
14(19):
6207 - 6217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. H. Martin, P. S. Woll, Z. Ni, J. C. Zuniga-Pflucker, and D. S. Kaufman
Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood-derived hematopoietic progenitor cells
Blood,
October 1, 2008;
112(7):
2730 - 2737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-M. Chan, S. Bonde, H. Klump, and N. Zavazava
Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells
Blood,
March 15, 2008;
111(6):
2953 - 2961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Qiu, E. N. Olivier, M. Velho, and E. E. Bouhassira
Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells
Blood,
February 15, 2008;
111(4):
2400 - 2408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. S. Woll, J. K. Morris, M. S. Painschab, R. K. Marcus, A. D. Kohn, T. L. Biechele, R. T. Moon, and D. S. Kaufman
Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells
Blood,
January 1, 2008;
111(1):
122 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Yu, M. A. Vodyanik, K. Smuga-Otto, J. Antosiewicz-Bourget, J. L. Frane, S. Tian, J. Nie, G. A. Jonsdottir, V. Ruotti, R. Stewart, et al.
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Science,
December 21, 2007;
318(5858):
1917 - 1920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Levenberg, J. Zoldan, Y. Basevitch, and R. Langer
Endothelial potential of human embryonic stem cells
Blood,
August 1, 2007;
110(3):
806 - 814.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kennedy, S. L. D'Souza, M. Lynch-Kattman, S. Schwantz, and G. Keller
Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures
Blood,
April 1, 2007;
109(7):
2679 - 2687.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Shinoda, K. Umeda, T. Heike, M. Arai, A. Niwa, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, R. Torii, et al.
{alpha}4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells
Blood,
March 15, 2007;
109(6):
2406 - 2415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bhatia
Hematopoietic Development from Human Embryonic Stem Cells
Hematology,
January 1, 2007;
2007(1):
11 - 16.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Vodyanik, J. A. Thomson, and I. I. Slukvin
Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures
Blood,
September 15, 2006;
108(6):
2095 - 2105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-H. Chang, A. M. Nelson, H. Cao, L. Wang, B. Nakamoto, C. B. Ware, and T. Papayannopoulou
Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin
Blood,
September 1, 2006;
108(5):
1515 - 1523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. E. Fleming and D. T. Scadden
Embryonic stem cells make human T cells
PNAS,
August 15, 2006;
103(33):
12213 - 12214.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Galic, S. G. Kitchen, A. Kacena, A. Subramanian, B. Burke, R. Cortado, and J. A. Zack
From the Cover: T lineage differentiation from human embryonic stem cells
PNAS,
August 1, 2006;
103(31):
11742 - 11747.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Lensch and G. Q. Daley
Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells
Blood,
April 1, 2006;
107(7):
2605 - 2612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. I. Slukvin, M. A. Vodyanik, J. A. Thomson, M. E. Gumenyuk, and K.-D. Choi
Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway.
J. Immunol.,
March 1, 2006;
176(5):
2924 - 2932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Narayan, J. L. Chase, R. L. Lewis, X. Tian, D. S. Kaufman, J. A. Thomson, and E. D. Zanjani
Human embryonic stem cell-derived hematopoietic cells are capable of engrafting primary as well as secondary fetal sheep recipients
Blood,
March 1, 2006;
107(5):
2180 - 2183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Olsen, D. L. Stachura, and M. J. Weiss
Designer blood: creating hematopoietic lineages from embryonic stem cells
Blood,
February 15, 2006;
107(4):
1265 - 1275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhang, K. Saeki, A. Kimura, K. Saeki, M. Nakahara, M. Doshi, Y. Kondo, T. Nakano, and A. Yuo
Efficient and Repetitive Production of Hematopoietic and Endothelial Cells from Feeder-Free Monolayer Culture System of Primate Embryonic Stem Cells
Biol Reprod,
February 1, 2006;
74(2):
295 - 306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Poggi, C. Prevosto, A.-M. Massaro, S. Negrini, S. Urbani, I. Pierri, R. Saccardi, M. Gobbi, and M. R. Zocchi
Interaction between Human NK Cells and Bone Marrow Stromal Cells Induces NK Cell Triggering: Role of NKp30 and NKG2D Receptors
J. Immunol.,
November 15, 2005;
175(10):
6352 - 6360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. S. Woll, C. H. Martin, J. S. Miller, and D. S. Kaufman
Human Embryonic Stem Cell-Derived NK Cells Acquire Functional Receptors and Cytolytic Activity
J. Immunol.,
October 15, 2005;
175(8):
5095 - 5103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-M. Zou, J.-J. Chen, M. C. Yoder, W. Wu, and J. D. Rowley
Knockdown of Pu.1 by small interfering RNA in CD34+ embryoid body cells derived from mouse ES cells turns cell fate determination to pro-B cells
PNAS,
September 13, 2005;
102(37):
13236 - 13241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. S. Ng, R. P. Davis, L. Azzola, E. G. Stanley, and A. G. Elefanty
Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation
Blood,
September 1, 2005;
106(5):
1601 - 1603.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. T. Zambidis, B. Peault, T. S. Park, F. Bunz, and C. I. Civin
Hematopoietic differentiation of human embryonic stem cells progresses through sequential hematoendothelial, primitive, and definitive stages resembling human yolk sac development
Blood,
August 1, 2005;
106(3):
860 - 870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wang, L. Li, P. Menendez, C. Cerdan, and M. Bhatia
Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development
Blood,
June 15, 2005;
105(12):
4598 - 4603.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Keller
Embryonic stem cell differentiation: emergence of a new era in biology and medicine
Genes & Dev.,
May 15, 2005;
19(10):
1129 - 1155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
