|
|
Blood, 15 January 2005, Vol. 105, No. 2, pp. 679-681.
Prepublished online as a Blood First Edition Paper on September 9, 2004; DOI 10.1182/blood-2004-05-1906.
 Previous Article | Table of Contents | Next Article 
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Brief report
Vascular leukocytes contribute to tumor vascularization
Jose R. Conejo-Garcia,
Ronald J. Buckanovich,
Fabian Benencia,
Maria C. Courreges,
Stephen C. Rubin,
Richard G. Carroll, and
George Coukos
From the Abramson Family Cancer Research Institute, the Center for Research in Reproduction and Women's Health, and the Division of Gynecologic Oncology, University of Pennsylvania, Philadelphia.
There is no proof that hematopoietic cells contribute significantly to vasculogenesis in postnatal life. Here we report a novel leukocyte subset within ovarian carcinoma that coexpresses endothelial and dendritic cell markers. Fluorescence-activated cell sorter (FACS) analysis identified a high frequency of VE-cadherin+ CD45+ leukocytes (39% of host cells) in 10 of 10 solid tumors evaluated. This population represented less than 1% of nontumor cells in ascites and peripheral blood. At the protein level, more than 86% of these cells expressed the endothelial markers P1H12, CD34, and CD31 and leukocyte markers CD11c and major histocompatibility complex (MHC) class II. At the mRNA level, we detected TEM1, TEM7, and Thy-1, specific markers of angiogenic endothelium. Finally, this population has the capacity to generate functional blood vessels in vivo. Because of its mixed phenotype, we named this population vascular leukocytes (VLCs). Our data provide an important link between hematopoietic endothelial precursors and vascular development in postnatal life and a possible novel therapeutic target.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. S. Kerbel
Tumor Angiogenesis
N. Engl. J. Med.,
May 8, 2008;
358(19):
2039 - 2049.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Grenier, A. Scime, F. Le Grand, A. Asakura, C. Perez-Iratxeta, M. A. Andrade-Navarro, P. A. Labosky, and M. A. Rudnicki
Resident Endothelial Precursors in Muscle, Adipose, and Dermis Contribute to Postnatal Vasculogenesis
Stem Cells,
December 1, 2007;
25(12):
3101 - 3110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Shaked and R. S. Kerbel
Antiangiogenic Strategies on Defense: On the Possibility of Blocking Rebounds by the Tumor Vasculature after Chemotherapy
Cancer Res.,
August 1, 2007;
67(15):
7055 - 7058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Nolan, A. Ciarrocchi, A. S. Mellick, J. S. Jaggi, K. Bambino, S. Gupta, E. Heikamp, M. R. McDevitt, D. A. Scheinberg, R. Benezra, et al.
Bone marrow-derived endothelial progenitor cells are a major determinant of nascent tumor neovascularization
Genes & Dev.,
June 15, 2007;
21(12):
1546 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Coukos
Monocytes TIE(2)d up in murky business
Blood,
June 15, 2007;
109(12):
5076 - 5076.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Venneri, M. D. Palma, M. Ponzoni, F. Pucci, C. Scielzo, E. Zonari, R. Mazzieri, C. Doglioni, and L. Naldini
Identification of proangiogenic TIE2-expressing monocytes (TEMs) in human peripheral blood and cancer
Blood,
June 15, 2007;
109(12):
5276 - 5285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Bak, J. J. Walters, M. Takeya, J. R. Conejo-Garcia, and B. L. Berwin
Scavenger Receptor-A-Targeted Leukocyte Depletion Inhibits Peritoneal Ovarian Tumor Progression
Cancer Res.,
May 15, 2007;
67(10):
4783 - 4789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Buckanovich, D. Sasaroli, A. O'Brien-Jenkins, J. Botbyl, R. Hammond, D. Katsaros, R. Sandaltzopoulos, L. A. Liotta, P. A. Gimotty, and G. Coukos
Tumor Vascular Proteins As Biomarkers in Ovarian Cancer
J. Clin. Oncol.,
March 1, 2007;
25(7):
852 - 861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Webster, E. H. Ekland, L. M. Agle, S. Chyou, R. Ruggieri, and T. T. Lu
Regulation of lymph node vascular growth by dendritic cells
J. Exp. Med.,
August 7, 2006;
203(8):
1903 - 1913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Cao, R. C. Savani, M. Fehrenbach, C. Lyons, L. Zhang, G. Coukos, and H. M. DeLisser
Involvement of Endothelial CD44 during in Vivo Angiogenesis
Am. J. Pathol.,
July 1, 2006;
169(1):
325 - 336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Li, E. E. Sharpe, A. B. Maupin, A. A. Teleron, A. L. Pyle, P. Carmeliet, and P. P. Young
VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization
FASEB J,
July 1, 2006;
20(9):
1495 - 1497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. E. Sharpe III, A. A. Teleron, B. Li, J. Price, M. S. Sands, K. Alford, and P. P. Young
The Origin and in Vivo Significance of Murine and Human Culture-Expanded Endothelial Progenitor Cells
Am. J. Pathol.,
May 1, 2006;
168(5):
1710 - 1721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Duda, K. S. Cohen, S. V. Kozin, J. Y. Perentes, D. Fukumura, D. T. Scadden, and R. K. Jain
Evidence for incorporation of bone marrow-derived endothelial cells into perfused blood vessels in tumors
Blood,
April 1, 2006;
107(7):
2774 - 2776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Anghelina, P. Krishnan, L. Moldovan, and N. I. Moldovan
Monocytes/Macrophages Cooperate with Progenitor Cells during Neovascularization and Tissue Repair: Conversion of Cell Columns into Fibrovascular Bundles
Am. J. Pathol.,
February 1, 2006;
168(2):
529 - 541.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
