|
|
Blood, 15 January 2005, Vol. 105, No. 2, pp. 742-749.
Prepublished online as a Blood First Edition Paper on September 21, 2004; DOI 10.1182/blood-2004-05-1891.
 Previous Article | Table of Contents | Next Article 
IMMUNOBIOLOGY
Selective Rac1 inhibition in dendritic cells diminishes apoptotic cell uptake and cross-presentation in vivo
Kristen M. Kerksiek,
Florence Niedergang,
Philippe Chavrier,
Dirk H. Busch, and
Thomas Brocker
From the Institute for Immunology, Ludwig-Maximilians University, Munich, Germany; Membrane and Cytoskeleton Dynamics Group, Institut Curie, Centre National de la Recherche Scientifique UMR-144, Paris, France; and Institute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany.
To better understand the influence of cytoskeletal regulation on dendritic cell (DC) function in vivo, the Rho guanosine triphosphatase (GTPase) Rac1 was selectively inhibited in DCs in transgenic (Tg) mice. Although transgene expression did not interfere with the migratory capacities of DC in vivo, a decreased uptake of fluorescent probes was observed. Interestingly, the absence of full Rac1 function most strongly affected the development and function of CD8+ DCs. Apoptotic cell uptake was severely reduced in Tg mice, impairing subsequent DC-mediated cross-presentation and priming of bacteria-specific T-cell responses. These findings highlight a special role for Rac1 in the capacity of CD8+ DCs to endocytose apoptotic cells and prime T cells via cross-presentation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
Dendritic cells thrive on Rac1
- Paige Lacy
Blood 2005 105: 433.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
A. G. Dupuy and E. Caron
Integrin-dependent phagocytosis - spreading from microadhesion to new concepts
J. Cell Sci.,
June 1, 2008;
121(11):
1773 - 1783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Werner-Klein, C. Dresch, P. Marconi, and T. Brocker
Transcriptional Targeting of B Cells for Induction of Peripheral CD8 T Cell Tolerance
J. Immunol.,
June 15, 2007;
178(12):
7738 - 7746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. L. Tourkova, G. V. Shurin, S. Wei, and M. R. Shurin
Small Rho GTPases Mediate Tumor-Induced Inhibition of Endocytic Activity of Dendritic Cells
J. Immunol.,
June 15, 2007;
178(12):
7787 - 7793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sethi, K. M. Kerksiek, T. Brocker, and H. Kretzschmar
Role of the CD8+ Dendritic Cell Subset in Transmission of Prions
J. Virol.,
May 1, 2007;
81(9):
4877 - 4880.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mattei, G. Schiavoni, P. Borghi, M. Venditti, I. Canini, P. Sestili, I. Pietraforte, H. C. Morse III, C. Ramoni, F. Belardelli, et al.
ICSBP/IRF-8 differentially regulates antigen uptake during dendritic-cell development and affects antigen presentation to CD4+ T cells
Blood,
July 15, 2006;
108(2):
609 - 617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Schnorrer, G. M. N. Behrens, N. S. Wilson, J. L. Pooley, C. M. Smith, D. El-Sukkari, G. Davey, F. Kupresanin, M. Li, E. Maraskovsky, et al.
The dominant role of CD8+ dendritic cells in cross-presentation is not dictated by antigen capture
PNAS,
July 11, 2006;
103(28):
10729 - 10734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. T. Buhler, C. A. Wiedig, Y. Schmid, G. A. Grassl, E. Bohn, and I. B. Autenrieth
The Yersinia enterocolitica Invasin Protein Promotes Major Histocompatibility Complex Class I- and Class II-Restricted T-Cell Responses
Infect. Immun.,
July 1, 2006;
74(7):
4322 - 4329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Lauterbach, A. Gruber, C. Ried, C. Cheminay, and T. Brocker
Insufficient APC Capacities of Dendritic Cells in Gene Gun-Mediated DNA Vaccination.
J. Immunol.,
April 15, 2006;
176(8):
4600 - 4607.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
