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Blood, 1 February 2005, Vol. 105, No. 3, pp. 1010-1015. Prepublished online as a Blood First Edition Paper on September 21, 2004; DOI 10.1182/blood-2004-04-1498. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-04-1498v1 105/3/1010    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Laukkanen, M. O. Articles by Dunbar, C. E. Search for Related Content PubMed PubMed Citation Articles by Laukkanen, M. O. Articles by Dunbar, C. E. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Low-dose total body irradiation causes clonal fluctuation of primate hematopoietic stem and progenitor cells Mikko O. Laukkanen, Ken Kuramoto, Boris Calmels, Masaaki Takatoku, Christof von Kalle, Robert E. Donahue, and Cynthia E. Dunbar From the Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD; and the Division of Experimental Hematology, Children's Research Foundation, Cincinnati Children's Hospital Medical Center, Cincinnati, OH. Due to high frequency of side effects caused by high-dose total body irradiation (TBI) the nonmyeloablative regimen together with cytotoxic agents is currently used especially for elderly patients. However, immediate and long-term effects of low-dose irradiation used in allogeneic transplantation on stem cells is less well known. We have studied the effect of low-dose 3 Gy TBI on the number of hematopoietic stem cell (HSC) clones contributing simultaneously to granulocyte production in rhesus macaque. The number of clones after 3 Gy TBI decreased markedly by 2 to 3 weeks after 3 Gy TBI, followed by a period of clonal instability, and recovery to almost pre–3 Gy TBI clonal diversity. The clones accounting for this recovery contributed before 3 Gy TBI, suggesting the profound initial impact of TBI was on a pool of progenitor cells, whereas most of the more primitive HSCs remained unaffected and were able to again contribute to hematopoiesis after recovery. Clonal fluctuation may indirectly suggest the presence of short-term/long-term HSC populations in rhesus macaque bone marrow as reported in a mouse model. The results indicate that even low-dose irradiation affects hematopoietic clonal dynamics and have implications for design of conditioning regimens for transplantation purposes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Yahata, Y. Muguruma, S. Yumino, Y. Sheng, T. Uno, H. Matsuzawa, M. Ito, S. Kato, T. Hotta, and K. Ando Quiescent Human Hematopoietic Stem Cells in the Bone Marrow Niches Organize the Hierarchical Structure of Hematopoiesis Stem Cells, December 1, 2008; 26(12): 3228 - 3236. [Abstract] [Full Text] [PDF] T. R. Bauer Jr, M. Hai, L. M. Tuschong, T. H. Burkholder, Y.-c. Gu, R. A. Sokolic, C. Ferguson, C. E. Dunbar, and D. D. Hickstein Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy Blood, November 15, 2006; 108(10): 3313 - 3320. [Abstract] [Full Text] [PDF] B. Heissig, S. Rafii, H. Akiyama, Y. Ohki, Y. Sato, T. Rafael, Z. Zhu, D. J. Hicklin, K. Okumura, H. Ogawa, et al. Low-dose irradiation promotes tissue revascularization through VEGF release from mast cells and MMP-9-mediated progenitor cell mobilization J. Exp. Med., September 19, 2005; 202(6): 739 - 750. [Abstract] [Full Text] [PDF]

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