Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
Blood, 1 February 2005, Vol. 105, No. 3, pp. 1068-1077.
Prepublished online as a Blood First Edition Paper on September 23, 2004; DOI 10.1182/blood-2004-03-1051.


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-03-1051v1
105/3/1068    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tepper, O. M.
Right arrow Articles by Gurtner, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tepper, O. M.
Right arrow Articles by Gurtner, G. C.
Related Collections
Right arrow Hematopoiesis and Stem Cells
Right arrow Hemostasis, Thrombosis, and Vascular Biology
Right arrow Cell Adhesion and Motility
Right arrowRelated Article in Blood Online
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY

Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone marrow–derived cells

Oren M. Tepper, Jennifer M. Capla, Robert D. Galiano, Daniel J. Ceradini, Matthew J. Callaghan, Mark E. Kleinman, and Geoffrey C. Gurtner

From The Laboratory of Microvascular Research and Vascular Tissue Engineering, New York University Medical Center, New York, NY.

Ischemia is a known stimulus for vascular growth. Bone marrow (BM)–derived endothelial progenitor cells (EPCs) are believed to contribute to new blood vessel growth, but the mechanism for this contribution is unknown. To elucidate how BM cells are able to form new blood vessels, a novel murine model of soft tissue ischemia was developed in lethally irradiated mice with BM reconstituted from either tie2/lacZ or ROSA/green fluorescent protein (GFP) mice (n = 24). BM-derived EPCs were recruited to ischemic tissue within 72 hours, and the extent of recruitment was directly proportional to the degree of tissue ischemia. At 7 days, there were persistently elevated levels of vascular endothelial growth factor (VEGF) (2.5-fold) and circulating VEGF receptor-2/CD11 (flk-1+/CD11) cells (18-fold) which correlated with increased numbers of BM-derived EPCs within ischemic tissue. The cells were initially located extravascularly as proliferative clusters. By day 14, these clusters coalesced into vascular cords, which became functional vessels by day 21. In vitro examination of human EPCs from healthy volunteers (n = 10) confirmed that EPC proliferation, adhesion, and chemotaxis were all significantly stimulated in hypoxic conditions. We conclude that BM-derived cells produce new blood vessels via localized recruitment, proliferation, and differentiation of circulating cells in a sequence of events markedly different from existing paradigms of angiogenesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article in Blood Online:

EPC: the ischemia warrior of the bone marrow
Edward W. Scott
Blood 2005 105: 918. [Full Text] [PDF]



This article has been cited by other articles:


Home page
Eur. J. Cardiothorac. Surg.Home page
N. Derval, L. Barandon, P. Dufourcq, L. Leroux, J.-M. D. Lamaziere, D. Daret, T. Couffinhal, and C. Duplaa
Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model.
Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 248 - 254.
[Abstract] [Full Text] [PDF]


Home page
Vasc MedHome page
C Kalka and I. Baumgartner
Gene and stem cell therapy in peripheral arterial occlusive disease
Vascular Medicine, May 1, 2008; 13(2): 157 - 172.
[Abstract] [PDF]


Home page
J. Biol. Chem.Home page
D. J. Ceradini, D. Yao, R. H. Grogan, M. J. Callaghan, D. Edelstein, M. Brownlee, and G. C. Gurtner
Decreasing Intracellular Superoxide Corrects Defective Ischemia-induced New Vessel Formation in Diabetic Mice
J. Biol. Chem., April 18, 2008; 283(16): 10930 - 10938.
[Abstract] [Full Text] [PDF]


Home page
PERSPECT VASC SURG ENDOVASC THERHome page
Miyamoto K, Nishigami K, Nagaya N, et al. Unblinded pilot study of autologous transplantation of bone marrow mononuclear cells in patients with thromboangiitis obliterans. Circulation. 2006;114: 2679-2684
Perspectives in Vascular Surgery and Endovascular Therapy, March 1, 2008; 20(1): 100 - 102.
[Abstract] [PDF]


Home page
DevelopmentHome page
U. Tigges, E. G. Hyer, J. Scharf, and W. B. Stallcup
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages
Development, February 1, 2008; 135(3): 523 - 532.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
J. Dulak, J. Deshane, A. Jozkowicz, and A. Agarwal
Heme Oxygenase-1 and Carbon Monoxide in Vascular Pathobiology: Focus on Angiogenesis
Circulation, January 15, 2008; 117(2): 231 - 241.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
E. I. Chang, S. A. Loh, D. J. Ceradini, E. I. Chang, S.-e Lin, N. Bastidas, S. Aarabi, D. A. Chan, M. L. Freedman, A. J. Giaccia, et al.
Age Decreases Endothelial Progenitor Cell Recruitment Through Decreases in Hypoxia-Inducible Factor 1{alpha} Stabilization During Ischemia
Circulation, December 11, 2007; 116(24): 2818 - 2829.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
M. E. Kleinman, M. R. Greives, S. S. Churgin, K. M. Blechman, E. I. Chang, D. J. Ceradini, O. M. Tepper, and G. C. Gurtner
Hypoxia-Induced Mediators of Stem/Progenitor Cell Trafficking Are Increased in Children With Hemangioma
Arterioscler. Thromb. Vasc. Biol., December 1, 2007; 27(12): 2664 - 2670.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
A. Kumin, M. Schafer, N. Epp, P. Bugnon, C. Born-Berclaz, A. Oxenius, A. Klippel, W. Bloch, and S. Werner
Peroxiredoxin 6 is required for blood vessel integrity in wounded skin
J. Cell Biol., November 19, 2007; 179(4): 747 - 760.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
W. B. Slayton, X.-M. Li, J. Butler, S. M. Guthrie, M. L. Jorgensen, J. R. Wingard, and E. W. Scott
The Role of the Donor in the Repair of the Marrow Vascular Niche Following Hematopoietic Stem Cell Transplant
Stem Cells, November 1, 2007; 25(11): 2945 - 2955.
[Abstract] [Full Text] [PDF]


Home page
Toxicol SciHome page
W. He, R. J. Greenwell, D. M. Brooks, L. Calderon-Garciduenas, H. D. Beall, and J. D. Coffin
Arsenic Exposure in Pregnant Mice Disrupts Placental Vasculogenesis and Causes Spontaneous Abortion
Toxicol. Sci., September 1, 2007; 99(1): 244 - 253.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
M. Egger, W. Schgoer, A. G. E. Beer, J. Jeschke, J. Leierer, M. Theurl, S. Frauscher, O. M. Tepper, A. Niederwanger, A. Ritsch, et al.
Hypoxia up-regulates the angiogenic cytokine secretoneurin via an HIF-1{alpha}- and basic FGF-dependent pathway in muscle cells
FASEB J, September 1, 2007; 21(11): 2906 - 2917.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
A. D. Hauer, G. H.M. van Puijvelde, N. Peterse, P. de Vos, V. van Weel, E. J.A. van Wanrooij, E. A.L. Biessen, P. H.A. Quax, A. G. Niethammer, R. A. Reisfeld, et al.
Vaccination Against VEGFR2 Attenuates Initiation and Progression of Atherosclerosis
Arterioscler. Thromb. Vasc. Biol., September 1, 2007; 27(9): 2050 - 2057.
[Abstract] [Full Text] [PDF]


Home page
HeartHome page
Y. Michowitz, E. Goldstein, D. Wexler, D. Sheps, G. Keren, and J. George
Circulating endothelial progenitor cells and clinical outcome in patients with congestive heart failure
Heart, September 1, 2007; 93(9): 1046 - 1050.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
J. Deshane, S. Chen, S. Caballero, A. Grochot-Przeczek, H. Was, S. Li Calzi, R. Lach, T. D. Hock, B. Chen, N. Hill-Kapturczak, et al.
Stromal cell-derived factor 1 promotes angiogenesis via a heme oxygenase 1-dependent mechanism
J. Exp. Med., March 19, 2007; 204(3): 605 - 618.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
G. C. Schatteman, M. Dunnwald, and C. Jiao
Biology of bone marrow-derived endothelial cell precursors
Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18.
[Abstract] [Full Text] [PDF]


Home page
HeartHome page
A Charest, A Pepin, R Shetty, C Cote, P Voisine, F Dagenais, P Pibarot, and P Mathieu
Distribution of SPARC during neovascularisation of degenerative aortic stenosis
Heart, December 1, 2006; 92(12): 1844 - 1849.
[Abstract] [Full Text] [PDF]


Home page
Vasc MedHome page
R. G. Shaffer, S. Greene, A. Arshi, G. Supple, A. Bantly, J. S Moores, M. S Parmacek, and E. R Mohler III
Effect of acute exercise on endothelial progenitor cells in patients with peripheral arterial disease
Vascular Medicine, November 1, 2006; 11(4): 219 - 226.
[Abstract] [PDF]


Home page
Am. J. Pathol.Home page
E. Kamoshita, Y. Ikeda, M. Fujita, H. Amano, A. Oikawa, T. Suzuki, Y. Ogawa, S. Yamashina, S. Azuma, S. Narumiya, et al.
Recruitment of a Prostaglandin E Receptor Subtype, EP3-Expressing Bone Marrow Cells Is Crucial in Wound-Induced Angiogenesis
Am. J. Pathol., October 1, 2006; 169(4): 1458 - 1472.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
L. J. Goldstein, K. A. Gallagher, S. M. Bauer, R. J. Bauer, V. Baireddy, Z.-J. Liu, D. G. Buerk, S. R. Thom, and O. C. Velazquez
Endothelial Progenitor Cell Release into Circulation Is Triggered by Hyperoxia-Induced Increases in Bone Marrow Nitric Oxide
Stem Cells, October 1, 2006; 24(10): 2309 - 2318.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
B. Li, E. E. Sharpe, A. B. Maupin, A. A. Teleron, A. L. Pyle, P. Carmeliet, and P. P. Young
VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization
FASEB J, July 1, 2006; 20(9): 1495 - 1497.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
L. Pardanaud and A. Eichmann
Identification, emergence and mobilization of circulating endothelial cells or progenitors in the embryo
Development, July 1, 2006; 133(13): 2527 - 2537.
[Abstract] [Full Text] [PDF]


Home page
aacredbookHome page
S. Rafii and D. Lyden
Contribution of Hematopoietic and Vascular Progenitor Cells to the Neoangiogenic Niche
Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 181 - 185.
[Full Text] [PDF]


Home page
Am. J. Pathol.Home page
M. Anghelina, P. Krishnan, L. Moldovan, and N. I. Moldovan
Monocytes/Macrophages Cooperate with Progenitor Cells during Neovascularization and Tissue Repair: Conversion of Cell Columns into Fibrovascular Bundles
Am. J. Pathol., February 1, 2006; 168(2): 529 - 541.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
J. J. Minguell and A. Erices
Mesenchymal Stem Cells and the Treatment of Cardiac Disease
Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
N. Landazuri and W. R. Taylor
The stem cell shell game. Focus on "The postnatal rat aorta contains pericyte progenitor cells that form spheroidal colonies in suspension culture"
Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1361 - C1362.
[Full Text] [PDF]



 click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020