SEARCH:   Advanced

Blood, 15 February 2005, Vol. 105, No. 4, pp. 1699-1705. Prepublished online as a Blood First Edition Paper on October 7, 2004; DOI 10.1182/blood-2004-06-2485. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-06-2485v1 105/4/1699    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xu, M. Articles by Hoffman, R. Search for Related Content PubMed PubMed Citation Articles by Xu, M. Articles by Hoffman, R. Related Collections Hematopoiesis and Stem Cells Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA The constitutive mobilization of bone marrow-repopulating cells into the peripheral blood in idiopathic myelofibrosis Mingjiang Xu, Edward Bruno, Joseph Chao, Hongyu Ni, Valerie Lindgren, Rafael Nunez, Nadim Mahmud, Guido Finazzi, Steven M. Fruchtman, Uday Popat, Enli Liu, Josef T. Prchal, Damiano Rondelli, Giovanni Barosi, and Ronald Hoffman From the Section of Hematology/Oncology and the Department of Pathology, University of Illinois at Chicago Cancer Center, University of Illinois College of Medicine, Chicago, IL; the Department of Hematology, Ospedali Riuniti, Bergamo, Italy; Mount Sinai School of Medicine, New York, NY; Baylor College of Medicine, Houston, TX; Instituto di Ricovere Cura a Corattere Scientifico (IRCCS) Policlinico S Matteo, Pavia, Italy; and The MPD Research Consortium, Chicago, IL. Idiopathic myelofibrosis (IM) is characterized by the constitutive mobilization of CD34+ cells. IM peripheral blood (PB) CD34+ cells had a reduced cloning efficiency and a lower frequency of cobblestone areas compared with normal granulocyte colony-stimulating factor (G-CSF)-mobilized PB CD34+ cells. IM CD34+ cells engrafted nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice, demonstrating that they contain bone marrow (BM)-repopulating cells. G-CSF-mobilized CD34+ cells produced multiple hematopoietic lineages within the NOD/SCID mice with a predominance of CD19+ cells. By contrast, IM CD34+ cells produced predominantly CD33+ cells, increased numbers of CD41+ cells, but fewer CD19+ cells. Transcriptional clonality assays of the engrafted human IM cells demonstrated their clonal origin. CD34+ cells from one patient isolated prior to leukemic transformation were capable of generating acute leukemia in NOD/SCID mice. The engrafted human cells exhibited the same abnormal karyotype as primary cells in a portion of the population. These findings demonstrate that BM-repopulating cells and more differentiated progenitor cells are constitutively mobilized into the PB in IM, and that their differentiation program is abnormal. In addition, the NOD/SCID model may be useful in gaining an understanding of the events occurring during the transition of IM to acute leukemia. (Blood. 2005;105:1699-1705) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Shi, Y. Zhao, T. Ishii, W. Hu, S. Sozer, W. Zhang, E. Bruno, V. Lindgren, M. Xu, and R. Hoffman Effects of Chromatin-Modifying Agents on CD34+ Cells from Patients with Idiopathic Myelofibrosis Cancer Res., July 1, 2007; 67(13): 6417 - 6424. [Abstract] [Full Text] [PDF] T. Nakayama, N. Mutsuga, and G. Tosato FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc Blood, February 15, 2007; 109(4): 1363 - 1372. [Abstract] [Full Text] [PDF] T. Nakayama, N. Mutsuga, and G. Tosato Effect of Fibroblast Growth Factor 2 on Stromal Cell-Derived Factor 1 Production by Bone Marrow Stromal Cells and Hematopoiesis J Natl Cancer Inst, February 7, 2007; 99(3): 223 - 235. [Abstract] [Full Text] [PDF] R. Hoffman and D. Rondelli Biology and Treatment of Primary Myelofibrosis Hematology, January 1, 2007; 2007(1): 346 - 354. [Abstract] [Full Text] [PDF] P. Guglielmelli, R. Zini, C. Bogani, S. Salati, A. Pancrazzi, E. Bianchi, F. Mannelli, S. Ferrari, M.-C. Le Bousse-Kerdiles, A. Bosi, et al. Molecular Profiling of CD34+ Cells in Idiopathic Myelofibrosis Identifies a Set of Disease-Associated Genes and Reveals the Clinical Significance of Wilms' Tumor Gene 1 (WT1) Stem Cells, January 1, 2007; 25(1): 165 - 173. [Abstract] [Full Text] [PDF] T. Ishii, E. Bruno, R. Hoffman, and M. Xu Involvement of various hematopoietic-cell lineages by the JAK2V617F mutation in polycythemia vera Blood, November 1, 2006; 108(9): 3128 - 3134. [Abstract] [Full Text] [PDF] F. Passamonti, E. Rumi, D. Pietra, M. G. D. Porta, E. Boveri, C. Pascutto, L. Vanelli, L. Arcaini, S. Burcheri, L. Malcovati, et al. Relation between JAK2 (V617F) mutation status, granulocyte activation, and constitutive mobilization of CD34+ cells into peripheral blood in myeloproliferative disorders Blood, May 1, 2006; 107(9): 3676 - 3682. [Abstract] [Full Text] [PDF] M. Xu, E. Bruno, J. Chao, S. Huang, G. Finazzi, S. M. Fruchtman, U. Popat, J. T. Prchal, G. Barosi, R. Hoffman, et al. Constitutive mobilization of CD34+ cells into the peripheral blood in idiopathic myelofibrosis may be due to the action of a number of proteases Blood, June 1, 2005; 105(11): 4508 - 4515. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020