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Blood, 1 April 2005, Vol. 105, No. 7, pp. 2764-2770. Prepublished online as a Blood First Edition Paper on December 16, 2004; DOI 10.1182/blood-2004-09-3567. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3567v1 105/7/2764    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Butenas, S. Articles by Mann, K. G. Search for Related Content PubMed PubMed Citation Articles by Butenas, S. Articles by Mann, K. G. Related Collections Hemostasis, Thrombosis, and Vascular Biology Phagocytes Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Tissue factor activity in whole blood Saulius Butenas, Beth A. Bouchard, Kathleen E. Brummel-Ziedins, Behnaz Parhami-Seren, and Kenneth G. Mann From the University of Vermont, Department of Biochemistry, Burlington, VT. Tissue factor (TF) is an integral membrane protein essential for hemostasis. During the past several years, a number of studies have suggested that physiologically active TF circulates in blood at concentrations greater than 30 pM either as a component of blood cells and microparticles or as a soluble plasma protein. In our studies using contact pathway–inhibited blood or plasma containing activated platelets, typically no clot is observed for 20 minutes in the absence of exogenous TF. An inhibitory anti-TF antibody also has no effect on the clotting time in the absence of exogenous TF. The addition of TF to whole blood at a concentration as low as 16 to 20 fM results in pronounced acceleration of clot formation. The presence of potential platelet TF activity was evaluated using ionophore-treated platelets and employing functional and immunoassays. No detectable TF activity or antigen was observed on quiescent or ionophore-stimulated platelets. Similarly, no TF antigen was detected on mononuclear cells in nonstimulated whole blood, whereas in lipopolysaccharide (LPS)–stimulated blood a significant fraction of monocytes express TF. Our data indicate that the concentration of physiologically active TF in non–cytokine-stimulated blood from healthy individuals cannot exceed and is probably lower than 20 fM. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Functional tissue factor in blood? Kenneth A. Bauer Blood 2005 105: 2620-2621. [Full Text] [PDF] This article has been cited by other articles: T. Orfeo, K. E. Brummel-Ziedins, M. Gissel, S. Butenas, and K. G. Mann The Nature of the Stable Blood Clot Procoagulant Activities J. Biol. Chem., April 11, 2008; 283(15): 9776 - 9786. [Abstract] [Full Text] [PDF] U. M. Okorie, W. S. Denney, M. S. Chatterjee, K. B. Neeves, and S. L. Diamond Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow Blood, April 1, 2008; 111(7): 3507 - 3513. [Abstract] [Full Text] [PDF] M. N. Diringer, B. E. Skolnick, S. A. Mayer, T. Steiner, S. M. Davis, N. C. Brun, and J. P. Broderick Risk of Thromboembolic Events in Controlled Trials of rFVIIa in Spontaneous Intracerebral Hemorrhage Stroke, March 1, 2008; 39(3): 850 - 856. [Abstract] [Full Text] [PDF] N. Mackman, R. E. Tilley, and N. S. 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Signal-dependent splicing of tissue factor pre-mRNA modulates the thrombogenecity of human platelets J. Exp. Med., October 30, 2006; 203(11): 2433 - 2440. [Abstract] [Full Text] [PDF] B. Uzonyi, K. Lotzer, S. Jahn, C. Kramer, M. Hildner, E. Bretschneider, D. Radke, M. Beer, R. Vollandt, J. F. Evans, et al. Cysteinyl leukotriene 2 receptor and protease-activated receptor 1 activate strongly correlated early genes in human endothelial cells PNAS, April 18, 2006; 103(16): 6326 - 6331. [Abstract] [Full Text] [PDF] M. A. Panteleev, M. V. Ovanesov, D. A. Kireev, A. M. Shibeko, E. I. Sinauridze, N. M. Ananyeva, A. A. Butylin, E. L. Saenko, and F. I. Ataullakhanov Spatial Propagation and Localization of Blood Coagulation Are Regulated by Intrinsic and Protein C Pathways, Respectively Biophys. J., March 1, 2006; 90(5): 1489 - 1500. [Abstract] [Full Text] [PDF] J. Steffel, T. F. Luscher, and F. C. Tanner Tissue Factor in Cardiovascular Diseases: Molecular Mechanisms and Clinical Implications Circulation, February 7, 2006; 113(5): 722 - 731. [Abstract] [Full Text] [PDF] T. Orfeo, S. Butenas, K. E. Brummel-Ziedins, and K. G. Mann The Tissue Factor Requirement in Blood Coagulation J. Biol. Chem., December 30, 2005; 280(52): 42887 - 42896. [Abstract] [Full Text] [PDF] P. L. Gross, B. C. Furie, G. Merrill-Skoloff, J. Chou, and B. Furie Leukocyte-versus microparticle-mediated tissue factor transfer during arteriolar thrombus development J. Leukoc. Biol., December 1, 2005; 78(6): 1318 - 1326. [Abstract] [Full Text] [PDF] J. Jesty and E. Beltrami Positive Feedbacks of Coagulation: Their Role in Threshold Regulation Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2463 - 2469. [Abstract] [Full Text] [PDF] A. Malarstig, T. Tenno, N. Johnston, B. Lagerqvist, T. Axelsson, A.-C. Syvanen, L. Wallentin, and A. Siegbahn Genetic Variations in the Tissue Factor Gene Are Associated With Clinical Outcome in Acute Coronary Syndrome and Expression Levels in Human Monocytes Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2667 - 2672. [Abstract] [Full Text] [PDF] J. Niemetz, S. M. Day, N. Mackman, and W. P. Fay Tissue-factor-endowed leukocytes do cause thrombosis Blood, August 15, 2005; 106(4): 1506 - 1507. [Full Text] [PDF] E. M. Egorina, M. A. Sovershaev, G. Bjorkoy, F. X.E. Gruber, J. O. Olsen, B. Parhami-Seren, K. G. Mann, and B. Osterud Intracellular and Surface Distribution of Monocyte Tissue Factor: Application to Intersubject Variability Arterioscler. Thromb. Vasc. Biol., July 1, 2005; 25(7): 1493 - 1498. [Abstract] [Full Text] [PDF] I. Ott Soluble Tissue Factor Emerges From Inflammation Circ. Res., June 24, 2005; 96(12): 1217 - 1218. [Full Text] [PDF]

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