|
|
Blood, 1 April 2005, Vol. 105, No. 7, pp. 2852-2861.
Prepublished online as a Blood First Edition Paper on December 9, 2004; DOI 10.1182/blood-2004-09-3606.
 Previous Article | Table of Contents | Next Article 
IMMUNOBIOLOGY
Endothelial tetraspanin microdomains regulate leukocyte firm adhesion during extravasation
Olga Barreiro,
María Yáñez-Mó,
Mónica Sala-Valdés,
María Dolores Gutiérrez-López,
Susana Ovalle,
Adrian Higginbottom,
Peter N. Monk,
Carlos Cabañas, and
Francisco Sánchez-Madrid
From the Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain; Instituto de Farmacología y Toxicología, Consejo Superior de Investigaciones Científicas–Universidad Complutense de Madrid (CSIC-UCM), Facultad de Medicina, Universidad Complutense, Madrid, Spain; and Department of Academic Neurology, The Medical School, University of Sheffield, United Kingdom.
Tetraspanins associate with several transmembrane proteins forming microdomains involved in intercellular adhesion and migration. Here, we show that endothelial tetraspanins relocalize to the contact site with transmigrating leukocytes and associate laterally with both intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Alteration of endothelial tetraspanin microdomains by CD9–large extracellular loop (LEL)–glutathione S–transferase (GST) peptides or CD9/CD151 siRNA oligonucleotides interfered with ICAM-1 and VCAM-1 function, preventing lymphocyte transendothelial migration and increasing lymphocyte detachment under shear flow. Heterotypic intercellular adhesion mediated by VCAM-1 or ICAM-1 was augmented when expressed exogenously in the appropriate tetraspanin environment. Therefore, tetraspanin microdomains have a crucial role in the proper adhesive function of ICAM-1 and VCAM-1 during leukocyte adhesion and transendothelial migration.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Rohlena, O. L. Volger, J. D. van Buul, L. H.P. Hekking, J. M. van Gils, P. I. Bonta, R. D. Fontijn, J. A. Post, P. L. Hordijk, and A. J.G. Horrevoets
Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion
Cardiovasc Res,
January 1, 2009;
81(1):
187 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Baldwin, V. Novitskaya, R. Sadej, E. Pochec, A. Litynska, C. Hartmann, J. Williams, L. Ashman, J. A. Eble, and F. Berditchevski
Tetraspanin CD151 Regulates Glycosylation of {alpha}3{beta}1 Integrin
J. Biol. Chem.,
December 19, 2008;
283(51):
35445 - 35454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nakhaei-Nejad, A. M. Hussain, Q.-X. Zhang, and A. G. Murray
Endothelial PI 3-kinase activity regulates lymphocyte diapedesis
Am J Physiol Heart Circ Physiol,
December 1, 2007;
293(6):
H3608 - H3616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. van Buul, E. Kanters, and P. L. Hordijk
Endothelial Signaling by Ig-Like Cell Adhesion Molecules
Arterioscler. Thromb. Vasc. Biol.,
September 1, 2007;
27(9):
1870 - 1876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Pero, M. T. Borchers, K. Spicher, S. I. Ochkur, L. Sikora, S. P. Rao, H. Abdala-Valencia, K. R. O'Neill, H. Shen, M. P. McGarry, et al.
G{alpha}i2-mediated signaling events in the endothelium are involved in controlling leukocyte extravasation
PNAS,
March 13, 2007;
104(11):
4371 - 4376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gordon-Alonso, M. Yanez-Mo, O. Barreiro, S. Alvarez, M. A. Munoz-Fernandez, A. Valenzuela-Fernandez, and F. Sanchez-Madrid
Tetraspanins CD9 and CD81 Modulate HIV-1-Induced Membrane Fusion
J. Immunol.,
October 15, 2006;
177(8):
5129 - 5137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Cabrero, J. M. Serrador, O. Barreiro, M. Mittelbrunn, S. Naranjo-Suarez, N. Martin-Cofreces, M. Vicente-Manzanares, R. Mazitschek, J. E. Bradner, J. Avila, et al.
Lymphocyte Chemotaxis Is Regulated by Histone Deacetylase 6, Independently of Its Deacetylase Activity
Mol. Biol. Cell,
August 1, 2006;
17(8):
3435 - 3445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sala-Valdes, A. Ursa, S. Charrin, E. Rubinstein, M. E. Hemler, F. Sanchez-Madrid, and M. Yanez-Mo
EWI-2 and EWI-F Link the Tetraspanin Web to the Actin Cytoskeleton through Their Direct Association with Ezrin-Radixin-Moesin Proteins
J. Biol. Chem.,
July 14, 2006;
281(28):
19665 - 19675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Doulet, E. Donnadieu, M.-P. Laran-Chich, F. Niedergang, X. Nassif, P. O. Couraud, and S. Bourdoulous
Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures
J. Cell Biol.,
May 22, 2006;
173(4):
627 - 637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
