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Blood, 15 April 2005, Vol. 105, No. 8, pp. 3356-3364. Prepublished online as a Blood First Edition Paper on December 21, 2004; DOI 10.1182/blood-2004-08-3080. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-08-3080v1 105/8/3356    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cooling, L. L. W. Articles by Olson, J. D. Search for Related Content PubMed PubMed Citation Articles by Cooling, L. L. W. Articles by Olson, J. D. Related Collections Hemostasis, Thrombosis, and Vascular Biology Transfusion Medicine Transplantation Gene Expression Related Article in Blood Online Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Determinants of ABH expression on human blood platelets Laura L. W. Cooling, Kathleen Kelly, James Barton, Debbie Hwang, Theodore A. W. Koerner, and John D. Olson From the Department of Pathology, University of Michigan, Ann Arbor, MI; and the Department of Pathology, University of Iowa, Iowa City, IA. Platelets express ABH antigens, which can adversely effect platelet transfusion recovery and survival in ABH-incompatible recipients. To date, there has been no large, comprehensive study comparing specific donor factors with ABH expression on platelet membranes and glycoconjugates. We studied ABH expression in 166 group A apheresis platelet donors by flow cytometry, Western blotting, and thin layer chromatography relative to donor age, sex, A1/A2 subgroup, and Lewis phenotype. Overall, A antigen on platelet membranes, glycoproteins, and glycosphingolipids was linked to an A1 red blood cell (RBC) phenotype. Among A1 donors, platelet ABH varied significantly between donors (0%-87%). Intradonor variability, however, was minimal, suggesting that platelet ABH expression is a stable, donor-specific characteristic, with 5% of A1 donors typing as either ABH high- or low-expressers. Group A2 donors, in contrast, possessed a Bombay-like phenotype, lacking both A and H antigens. Unlike RBCs, ABH expression on platelets may be determined primarily by H-glycosyltransferase (FUT1) activity. Identification of A2 and A1 low expressers may increase the availability and selection of crossmatched and HLA-matched platelets. Platelets from group A2 may also be a superior product for patients undergoing A/O major mismatch allogeneic progenitor cell transplantation. (Blood. 2005;105:3356-3364) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Universal platelets: "A" change in dogma David Stroncek Blood 2005 105: 3008. [Full Text] [PDF] Related Letter in Blood Online: Expression of ABH antigens on platelets Brian R. Curtis, Richard H. Aster, and Laura L. Cooling Blood 2006 107: 842-843. [Full Text] [PDF] This article has been cited by other articles: T. R. Klumpp and D. L. Siegel Transfusion medicine ASH Self-Assessment Program, January 1, 2007; 2007(1): 408 - 443. [Full Text] [PDF] A. Nambiar, R. J. Duquesnoy, S. Adams, Y. Zhao, J. Oblitas, S. Leitman, D. Stroncek, and F. Marincola HLAMatchmaker-driven analysis of responses to HLA-typed platelet transfusions in alloimmunized thrombocytopenic patients Blood, February 15, 2006; 107(4): 1680 - 1687. [Abstract] [Full Text] [PDF] B. R. Curtis, R. H. Aster, and L. L. Cooling Expression of ABH antigens on platelets Blood, January 15, 2006; 107(2): 842 - 843. [Full Text] [PDF]

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