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Blood, 1 May 2005, Vol. 105, No. 9, pp. 3502-3511. Prepublished online as a Blood First Edition Paper on January 11, 2005; DOI 10.1182/blood-2004-09-3547. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3547v1 105/9/3502    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Patterson, L. J. Articles by Patient, R. Search for Related Content PubMed PubMed Citation Articles by Patterson, L. J. Articles by Patient, R. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Scl is required for dorsal aorta as well as blood formation in zebrafish embryos Lucy J. Patterson, Martin Gering, and Roger Patient From the Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom, and the Weatherall Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Headington, Oxford, United Kingdom. Blood and endothelial cells arise in close association in developing embryos, possibly from a shared precursor, the hemangioblast, or as hemogenic endothelium. The transcription factor, Scl/Tal1 (stem cell leukemia protein), is essential for hematopoiesis but thought to be required only for remodeling of endothelium in mouse embryos. By contrast, it has been implicated in hemangioblast formation in embryoid bodies. To resolve the role of scl in endothelial development, we knocked down its synthesis in zebrafish embryos where early precursors and later phenotypes can be more easily monitored. With respect to blood, the zebrafish morphants phenocopied the mouse knockout and positioned scl in the genetic hierarchy. Importantly, endothelial development was also clearly disrupted. Dorsal aorta formation was substantially compromised and gene expression in the posterior cardinal vein was abnormal. We conclude that scl is especially critical for the development of arteries where adult hematopoietic stem cells emerge, implicating scl in the formation of hemogenic endothelium. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Scl in the zebrafish: a simple (?) twist of fate Elaine Dzierzak Blood 2005 105: 3391-3392. 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