Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 May 2005, Vol. 105, No. 9, pp. 3691-3698.
Prepublished online as a Blood First Edition Paper on January 21, 2005; DOI 10.1182/blood-2003-07-2558.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2003-07-2558v1
105/9/3691    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, W.
Right arrow Articles by Lu, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, W.
Right arrow Articles by Lu, X.
Related Collections
Right arrow Neoplasia
Right arrow Cell Cycle
Right arrow Oncogenes and Tumor Suppressors
Right arrow Signal Transduction
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

NEOPLASIA

Posttranslational modifications of p27kip1 determine its binding specificity to different cyclins and cyclin-dependent kinases in vivo

Wenqing Zhang, Daniele Bergamaschi, Boquan Jin, and Xin Lu

From the Ludwig Institute for Cancer Research, University College London, London, United Kingdom; and the Department of Immunology, Fourth Military Medical University, Xi'an, China.

Using 2-dimensional gel electrophoresis (2D-gel) analysis, we show here that cell-cycle entry is associated with a significant increase in p27kip1 phosphorylation in human primary B cells. A similar pattern of increase in p27kip1 phosphorylation was also seen in 2 fast-growing tumor cell lines, Burkitt lymphoma cell line BL40 and breast carcinoma cell line Cal51, where inactive p27kip1 is expressed at high levels. Detailed analysis revealed for the first time that different cyclins and cyclin-dependent kinases (cdk's) interact with distinct posttranslationally modified isoforms of p27kip1 in vivo. Cyclin E but not cyclin A selectively interacts with phosphorylated p27kip1 isoforms, while cyclin D1 and D2 favor unphosphorylated p27kip1 isoforms in vivo. Interestingly, cyclin D3 and cdk4 selectively interact with phosphorylated p27kip1 in BL40 cells. Among all D-type cyclin/cdk4 and cdk6 complexes, cyclin D3/cdk4 is most active in sequestering the inhibitory activity of p27kip1 in vitro in a cyclinE/cdk2 kinase assay. This novel feature of the binding specificity of p27kip1 to cyclins and cdk's in vivo is interpreted in the context of overexpression of cyclin D3 in the presence of high levels of p27kip1 in human B-cell lymphomas with adverse clinical outcome.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
J.-W. Cui, Y.-J. Li, A. Sarkar, J. Brown, Y.-H. Tan, M. Premyslova, C. Michaud, N. Iscove, G.-J. Wang, and Y. Ben-David
Retroviral insertional activation of the Fli-3 locus in erythroleukemias encoding a cluster of microRNAs that convert Epo-induced differentiation to proliferation
Blood, October 1, 2007; 110(7): 2631 - 2640.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
L. Hong, Y. Zhao, Y. Han, W. Guo, H. Jin, T. Qiao, Z. Che, and D. Fan
Mechanisms of growth arrest by zinc ribbon domain-containing 1 in gastric cancer cells
Carcinogenesis, August 1, 2007; 28(8): 1622 - 1628.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Susaki, K. Nakayama, and K. I. Nakayama
Cyclin D2 Translocates p27 out of the Nucleus and Promotes Its Degradation at the G0-G1 Transition
Mol. Cell. Biol., July 1, 2007; 27(13): 4626 - 4640.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. Pyndiah, J. P. Lasserre, A. Menard, S. Claverol, V. Prouzet-Mauleon, F. Megraud, F. Zerbib, and M. Bonneu
Two-dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes from Helicobacter pylori
Mol. Cell. Proteomics, February 1, 2007; 6(2): 193 - 206.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. El Wakil, C. Francius, A. Wolff, J. Pleau-Varet, and J. Nardelli
The GATA2 transcription factor negatively regulates the proliferation of neuronal progenitors
Development, June 1, 2006; 133(11): 2155 - 2165.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020