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Blood, 1 July 2005, Vol. 106, No. 1, pp. 216-223. Prepublished online as a Blood First Edition Paper on March 24, 2005; DOI 10.1182/blood-2005-01-0220. This Article Full Text Full Text (PDF) Supplemental Figures and Table All Versions of this Article: 2005-01-0220v1 106/1/216    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Segura, E. Articles by Théry, C. Search for Related Content PubMed PubMed Citation Articles by Segura, E. Articles by Théry, C. 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Since immature and mature DCs induce different functional T-cell responses (ie, tolerance versus priming), we asked whether DC maturation also influenced the priming abilities of their exosomes. We show that exosomes secreted by lipopolysaccharide (LPS)–treated mature DCs are 50- to 100-fold more potent to induce antigen-specific T-cell activation in vitro than exosomes from immature DCs. In vitro, exosomes from mature DCs transfer to B lymphocytes the ability to prime naive T cells. In vivo, only mature exosomes trigger effector T-cell responses, leading to fast skin graft rejection. Proteomic and biochemical analyses revealed that mature exosomes are enriched in MHC class II, B7.2, intercellular adhesion molecule 1 (ICAM-1), and bear little milk-fat globule–epidermal growth factor–factor VIII (MFG-E8) as compared with immature exosomes. Functional analysis using DC-derived exosomes from knock-out mice showed that MHC class II and ICAM-1 are required for mature exosomes to prime naive T cells, whereas B7.2 and MFG-E8 are dispensable. Therefore, changes in protein composition and priming abilities of exosomes reflect the maturation signals received by DCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. K. Khatua, H. E. Taylor, J. E. K. Hildreth, and W. Popik Exosomes Packaging APOBEC3G Confer Human Immunodeficiency Virus Resistance to Recipient Cells J. Virol., January 15, 2009; 83(2): 512 - 521. [Abstract] [Full Text] [PDF] N. Prado, E. G. Marazuela, E. Segura, H. Fernandez-Garcia, M. Villalba, C. Thery, R. Rodriguez, and E. Batanero Exosomes from Bronchoalveolar Fluid of Tolerized Mice Prevent Allergic Reaction J. Immunol., July 15, 2008; 181(2): 1519 - 1525. [Abstract] [Full Text] [PDF] A. Clayton, J. P. Mitchell, J. Court, S. 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