Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 July 2005, Vol. 106, No. 1, pp. 304-310.
Prepublished online as a Blood First Edition Paper on March 10, 2005; DOI 10.1182/blood-2005-01-0241.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Table
Right arrow All Versions of this Article:
2005-01-0241v1
106/1/304    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chou, W.-C.
Right arrow Articles by Dang, C. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chou, W.-C.
Right arrow Articles by Dang, C. V.
Related Collections
Right arrow Neoplasia
Right arrow Signal Transduction
Right arrow Gene Expression
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

NEOPLASIA

Arsenic suppresses gene expression in promyelocytic leukemia cells partly through Sp1 oxidation

Wen-Chien Chou, Hsuan-Yu Chen, Sung-Liang Yu, Linzhao Cheng, Pan-Chyr Yang, and Chi V. Dang

From the Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei; Graduate Institute of Epidemiology, National Taiwan University, Taipei; and the Department of Medicine, Program of Human Genetics and Molecular Biology, Institute for Cell Engineering, and the Kimmel Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD.

The mechanism by which arsenic dramatically affects gene expression remains poorly understood. Here we report that prolonged exposure of acute promyelocytic leukemia NB4 cells to low levels of arsenic trioxide increased the expression of a set of genes responsible for reactive oxygen species (ROS) production. We hypothesize that arsenic-induced ROS in turn contribute partially to altered gene expression. To identify genes responsive to arsenic-induced ROS, we used microarray gene expression analysis and identified genes that responded to arsenic and hydrogen peroxide but whose response to arsenic was reversed by an ROS scavenger, N-acetyl-L-cysteine. We found that 26% of the genes significantly responsive to arsenic might have been directly altered by ROS. We further explored the mechanisms by which ROS affects gene regulation and found that the Sp1 transcription factor was oxidized by arsenic treatment, with a corresponding decrease in its in situ binding on the promoters of 3 genes, hTERT, C17, and c-Myc, whose expressions were significantly suppressed. We conclude that ROS contributed partly to arsenic-mediated gene regulation and that Sp1 oxidation contributed to gene suppression by arsenic-induced ROS.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
G.-M. Zou and A. Maitra
Small-molecule inhibitor of the AP endonuclease 1/REF-1 E3330 inhibits pancreatic cancer cell growth and migration
Mol. Cancer Ther., July 1, 2008; 7(7): 2012 - 2021.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
T. Mijatovic, N. De Neve, P. Gailly, V. Mathieu, B. Haibe-Kains, G. Bontempi, J. Lapeira, C. Decaestecker, V. Facchini, and R. Kiss
Nucleolus and c-Myc: potential targets of cardenolide-mediated antitumor activity
Mol. Cancer Ther., May 1, 2008; 7(5): 1285 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Z.-Y. Wang and Z. Chen
Acute promyelocytic leukemia: from highly fatal to highly curable
Blood, March 1, 2008; 111(5): 2505 - 2515.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. H. Lam, C. L. Winata, Y. Tong, S. Korzh, W. S. Lim, V. Korzh, J. Spitsbergen, S. Mathavan, L. D. Miller, E. T. Liu, et al.
Transcriptome kinetics of arsenic-induced adaptive response in zebrafish liver
Physiol Genomics, November 21, 2006; 27(3): 351 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
M. Argos, M. G. Kibriya, F. Parvez, F. Jasmine, M. Rakibuz-Zaman, and H. Ahsan
Gene expression profiles in peripheral lymphocytes by arsenic exposure and skin lesion status in a bangladeshi population.
Cancer Epidemiol. Biomarkers Prev., July 1, 2006; 15(7): 1367 - 1375.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020