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Blood, 1 July 2005, Vol. 106, No. 1, pp. 51-58. Prepublished online as a Blood First Edition Paper on March 17, 2005; DOI 10.1182/blood-2004-11-4491. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-11-4491v1 106/1/51    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lucas, M. L. Articles by Bodine, D. M. Search for Related Content PubMed PubMed Citation Articles by Lucas, M. L. Articles by Bodine, D. M. Related Collections Free Research Articles Gene Therapy Hematopoiesis Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  GENE THERAPY Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopes M. Lee Lucas, Nancy E. Seidel, Christopher D. Porada, John G. Quigley, Stacie M. Anderson, Harry L. Malech, Janis L. Abkowitz, Esmail D. Zanjani, and David M. Bodine From the Genetics and Molecular Biology Branch (GMBB), National Human Genome Research Institute (NHGRI), National Institutes of Health (NIH), Bethesda, MD; University of Nevada, Reno; National Institute of Allergy and Infectious Diseases (NIAID), NIH, Bethesda, MD; and University of Washington, Seattle. Gene therapy for hematopoietic diseases has been hampered by the low frequency of transduction of human hematopoietic stem cells (HSCs) with retroviral vectors pseudotyped with amphotropic envelopes. We hypothesized that transduction could be increased by the use of retroviral vectors pseudotyped with envelopes that recognize more abundant cellular receptors. The levels of mRNA encoding the receptors of the feline retroviruses, RD114 and feline leukemia virus type C (FeLV-C), were significantly higher than the level of gibbon ape leukemia virus (GaLV) receptor mRNA in cells enriched for human HSCs (Lin– CD34+ CD38–). We cotransduced human peripheral blood CD34+ cells with equivalent numbers of FeLV-C and GALV or RD114 and GALV-pseudotyped retroviruses for injection into fetal sheep. Analysis of DNA from peripheral blood and bone marrow from recipient sheep demonstrated that FeLV-C– or RD114-pseudotyped vectors were present at significantly higher levels than GALV-pseudotyped vectors. Analysis of individual myeloid colonies demonstrated that retrovirus vectors with FeLV-C and RD114 pseudotypes were present at 1.5 to 1.6 copies per cell and were preferentially integrated near known genes We conclude that the more efficient transduction of human HSCs with either FeLV-C– or RD114-pseudotyped retroviral particles may improve gene transfer in human clinical trials. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Improved human stem-cell transduction: the cat's meow Brian P. Sorrentino Blood 2005 106: 8. [Full Text] [PDF] This article has been cited by other articles: A. W. NIENHUIS Eighth Cooley's Anemia Symposium: Summation and Perspective Ann. N.Y. Acad. Sci., November 1, 2005; 1054(1): 396 - 406. [Abstract] [Full Text] [PDF]

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