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Blood, 1 July 2005, Vol. 106, No. 1, pp. 95-102. Prepublished online as a Blood First Edition Paper on March 24, 2005; DOI 10.1182/blood-2004-09-3652. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3652v1 106/1/95    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Storms, R. W. Articles by Smith, C. A. Search for Related Content PubMed PubMed Citation Articles by Storms, R. W. Articles by Smith, C. A. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34 Robert W. Storms, Patrick D. Green, Kristine M. Safford, Donna Niedzwiecki, Christopher R. Cogle, O. Michael Colvin, Nelson J. Chao, Henry E. Rice, and Clayton A. Smith From the Departments of Medicine, Surgery, and Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC; and the Department of Hematology, University of British Columbia, Vancouver, BC, Canada. A broad range of hematopoietic stem cells and progenitors reside within a fraction of umbilical cord blood (UCB) that exhibits low light scatter properties (SSClo) and high expression of aldehyde dehydrogenase (ALDHbr). Many SSClo ALDHbr cells coexpress CD34; however, other cells express either ALDH or CD34. To investigate the developmental potential of these cell subsets, purified ALDHbr CD34+, ALDHneg CD34+, and ALDHbr CD34neg UCB cells were characterized within a variety of in vivo and in vitro assays. Primitive progenitors capable of multilineage development were monitored in long- and short-term repopulation assays performed on nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, and in primary and secondary long-term culture assays. These progenitors were highly enriched within the ALDHbr CD34+ fraction. This cell fraction also enriched short-term myeloid progenitors that were detected in vitro. By comparison, ALDHneg CD34+ cells contained few primitive progenitors and had diminished short-term myeloid potential but exhibited enhanced short-term natural killer (NK) cell development in vitro. The ALDHbr CD34neg cells were not efficiently supported by any of the assays used. These studies suggested that in particular the expression of ALDH delineated distinct CD34+ stem cell and progenitor compartments. The differential expression of ALDH may provide a means to explore normal and malignant processes associated with myeloid and lymphoid development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. S. Hart and W. S. El-Deiry Invincible, but Not Invisible: Imaging Approaches Toward In Vivo Detection of Cancer Stem Cells J. Clin. Oncol., June 10, 2008; 26(17): 2901 - 2910. [Abstract] [Full Text] [PDF] D. A. Hess, T. P. Craft, L. Wirthlin, S. Hohm, P. Zhou, W. C. Eades, M. H. Creer, M. S. Sands, and J. A. Nolta Widespread Nonhematopoietic Tissue Distribution by Transplanted Human Progenitor Cells with High Aldehyde Dehydrogenase Activity Stem Cells, March 1, 2008; 26(3): 611 - 620. [Abstract] [Full Text] [PDF] T. J. Povsic, K. L. Zavodni, F. L. Kelly, S. Zhu, P. J. 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