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Blood, 15 November 2005, Vol. 106, No. 10, pp. 3490-3497. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2005-05-2044. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-05-2044v1 106/10/3490    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Penna, G. Articles by Adorini, L. Search for Related Content PubMed PubMed Citation Articles by Penna, G. Articles by Adorini, L. Related Collections Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3 Giuseppe Penna, Andrea Roncari, Susana Amuchastegui, Kenn C. Daniel, Emilio Berti, Marco Colonna, and Luciano Adorini From BioXell, Milano, Italy; Department of Dermatology, University of Milano-Bicocca and Ospedale Maggiore–Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Milano, Italy; and Department of Pathology and Immunology, Washinghton University School of Medicine, St Louis, MO. 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is a secosteroid hormone that renders dendritic cells (DCs) tolerogenic, favoring the induction of regulatory T cells. Induction of DCs with tolerogenic properties by 1,25(OH)2D3 is associated with increased selective expression of immunoglobulin-like transcript 3 (ILT3), suggesting its involvement in the immunoregulatory properties of this hormone. Here we show an in vivo correlate of the increased ILT3 expression on DCs in healing psoriatic lesions following topical treatment with the 1,25(OH)2D3 analog calcipotriol. Analysis of DC subsets reveals a differential regulation of ILT3 expression by 1,25(OH)2D3, with a marked up-regulation in myeloid DCs but no effect on its expression by plasmacytoid DCs. A regulatory role for ILT3 expressed on DCs is indicated by the increased interferon- (IFN-) secretion promoted by anti-ILT3 addition to cultures of DCs and T cells, but this effect is blunted in 1,25(OH)2D3-treated DCs, suggesting ILT3-independent mechanisms able to regulate T-cell activation. Although ILT3 expression by DCs is required for induction of regulatory T cells, DC pretreatment with 1,25(OH)2D3 leads to induction of CD4+Foxp3+ cells with suppressive activity irrespective of the presence of neutralizing anti-ILT3 monoclonal antibody (mAb), indicating that ILT3 expression is dispensable for the capacity of 1,25(OH)2D3-treated DCs to induce regulatory T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Brenk, M. Scheler, S. Koch, J. Neumann, O. Takikawa, G. Hacker, T. Bieber, and D. von Bubnoff Tryptophan Deprivation Induces Inhibitory Receptors ILT3 and ILT4 on Dendritic Cells Favoring the Induction of Human CD4+CD25+ Foxp3+ T Regulatory Cells J. Immunol., July 1, 2009; 183(1): 145 - 154. [Abstract] [Full Text] [PDF] M. Ghoreishi, P. Bach, J. Obst, M. Komba, J. C. Fleet, and J. P. 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