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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3691-3698. Prepublished online as a Blood First Edition Paper on August 16, 2005; DOI 10.1182/blood-2005-06-2326. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-06-2326v1 106/12/3691    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cheng, C. Articles by Krams, R. Search for Related Content PubMed PubMed Citation Articles by Cheng, C. Articles by Krams, R. Related Collections Hemostasis, Thrombosis, and Vascular Biology Plenary Papers Gene Expression Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLENARY PAPERS Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique Caroline Cheng, Rien van Haperen, Monique de Waard, Luc C. A. van Damme, Dennie Tempel, Laurens Hanemaaijer, Gert W. A. van Cappellen, Joop Bos, Cornelis J. Slager, Dirk J. Duncker, Anton F. W. van der Steen, Rini de Crom, and Rob Krams From the Departments of Cardiology, Thoraxcenter, Cell Biology and Genetics, Development and Reproduction, Experimental Medical Instrumentation, and Vascular Surgery, Erasmus University Medical Center, Rotterdam, The Netherlands. The focal location of atherosclerosis in the vascular tree is correlated with local variations in shear stress. We developed a method to induce defined variations in shear stress in a straight vessel segment of a mouse. To this end, a cylinder with a tapered lumen was placed around the carotid artery, inducing a high shear stress field. Concomitantly, regions of low shear stress and oscillatory shear stress were created upstream and down-stream of the device, respectively. This device was used in mice transgenic for an eNOS3GFP fusion gene. We observed a strong induction of endothelial nitric oxide synthase–green fluorescent protein (eNOS-GFP) mRNA expression in the high shear stress region compared with the other regions (P < .05). Quantification of eNOS-GFP fluorescence or of immunoreactivity to the Golgi complex or to platelet endothelial cell adhesion molecule 1 (PECAM-1) showed an increase in the high shear stress region (P < .05) compared with nontreated carotid arteries. Colocalization of eNOS-GFP with either the Golgi complex or PECAM-1 also responded to alterations of shear stress. In conclusion, we showed a direct response of mRNA and protein expression in vivo to induced variations of shear stress. This model provides the opportunity to study the relationship between shear stress alterations, gene expression, and atherosclerosis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Hemodynamic manipulation of eNOS in vivo Peter F. Davies Blood 2005 106: 3679-3680. [Full Text] [PDF] This article has been cited by other articles: C. Cheng, A. M. Noordeloos, V. Jeney, M. P. Soares, F. Moll, G. Pasterkamp, P. W. Serruys, and H. J. Duckers Heme Oxygenase 1 Determines Atherosclerotic Lesion Progression Into a Vulnerable Plaque Circulation, June 16, 2009; 119(23): 3017 - 3027. [Abstract] [Full Text] [PDF] J.-L. Balligand, O. Feron, and C. Dessy eNOS Activation by Physical Forces: From Short-Term Regulation of Contraction to Chronic Remodeling of Cardiovascular Tissues Physiol Rev, April 1, 2009; 89(2): 481 - 534. [Abstract] [Full Text] [PDF] M. E. Hartnett, D. Martiniuk, G. Byfield, P. Geisen, G. Zeng, and V. L. Bautch Neutralizing VEGF Decreases Tortuosity and Alters Endothelial Cell Division Orientation in Arterioles and Veins in a Rat Model of ROP: Relevance to Plus Disease Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 3107 - 3114. [Abstract] [Full Text] [PDF] L. Ai, M. Rouhanizadeh, J. C. Wu, W. Takabe, H. Yu, M. Alavi, R. Li, Y. Chu, J. Miller, D. D. Heistad, et al. Shear stress influences spatial variations in vascular Mn-SOD expression: implication for LDL nitration Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1576 - C1585. [Abstract] [Full Text] [PDF] N. Kleinstreuer, T. David, M. J. Plank, and Z. 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[Abstract] [Full Text] [PDF] Z Bagi, P Hamar, M Kardos, and A Koller Lack of flow mediated dilation and enhanced angiotensin II-induced constriction in skeletal muscle arterioles of lupus-prone autoimmune mice Lupus, June 1, 2006; 15(6): 326 - 334. [Abstract] [PDF] V. Gambillara, C. Chambaz, G. Montorzi, S. Roy, N. Stergiopulos, and P. Silacci Plaque-prone hemodynamics impair endothelial function in pig carotid arteries Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2320 - H2328. [Abstract] [Full Text] [PDF] Z.-G. Jin Where is endothelial nitric oxide synthase more critical: plasma membrane or Golgi? Arterioscler Thromb Vasc Biol, May 1, 2006; 26(5): 959 - 961. [Full Text] [PDF]

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