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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3803-3810. Prepublished online as a Blood First Edition Paper on August 11, 2005; DOI 10.1182/blood-2005-01-0179. This Article Full Text Full Text (PDF) Supplemental Videos All Versions of this Article: 2005-01-0179v1 106/12/3803    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lin, H.-F. Articles by Handin, R. I. Search for Related Content PubMed PubMed Citation Articles by Lin, H.-F. Articles by Handin, R. I. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Analysis of thrombocyte development in CD41-GFP transgenic zebrafish Hui-Feng Lin, David Traver, Hao Zhu, Kimberly Dooley, Barry H. Paw, Leonard I. Zon, and Robert I. Handin From the Hematology Division, Department of Medicine, Brigham & Women's Hospital, Boston; and the Hematology Division and Howard Hughes Medical Institute, Children's Hospital, Boston, MA. Thrombocytes are the nucleated equivalent of platelets in nonmammalian vertebrates such as the zebrafish, Danio rerio. We have cloned zebrafish CD41 cDNA (IIb, glycoprotein IIb [GPIIb]) and its promoter and have generated transgenic zebrafish lines with green fluorescent protein (GFP)–tagged thrombocytes. CD41 mRNA transcripts appeared 42 hours after fertilization (hpf) by reverse-transcriptase–polymerase chain reaction (RT-PCR) and at 48 hpf in circulating hematopoietic cells. Flow sorting of thrombocytes from the mesonephros of adult CD41-GFP zebrafish showed a GFPhigh subset, which had the morphologic appearance of mature thrombocytes, and a GFPlow subset with an immature appearance, suggesting that they may be thrombocyte precursors. Confocal laser microscopy of embryos 40 and 48 hpf also showed a nonmobile population of GFP+ cells in a discrete area between the dorsal aorta and caudal vein. Production of circulating thrombocytes was inhibited by the injection of antisense morpholinos for the stem-cell transcription factor scl and c-mpl, the receptor for thrombopoietin. The nonmobile pool of GFP+ cells was abolished by scl knockdown and partially inhibited by c-mpl knockdown. These studies have shown that it is possible to identify thrombocytes, thrombocyte precursors, and, possibly, early hematopoietic stem cells in zebrafish embryos and track their proliferation and maturation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A green light for the thrombopoietic program Pudur Jagadeeswaran Blood 2005 106: 3684. [Full Text] [PDF] This article has been cited by other articles: J. D. Amigo, G. E. Ackermann, J. J. Cope, M. Yu, J. D. Cooney, D. Ma, N. B. Langer, E. Shafizadeh, G. C. Shaw, W. Horsely, et al. The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish Blood, November 19, 2009; 114(21): 4654 - 4663. [Abstract] [Full Text] [PDF] E. van Rooijen, E. E. Voest, I. Logister, J. Korving, T. Schwerte, S. Schulte-Merker, R. H. Giles, and F. 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