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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3874-3879. Prepublished online as a Blood First Edition Paper on August 11, 2005; DOI 10.1182/blood-2005-03-0996. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-03-0996v1 106/12/3874    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carlin, L. M. Articles by Davis, D. M. Search for Related Content PubMed PubMed Citation Articles by Carlin, L. M. Articles by Davis, D. M. Related Collections Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Secretion of IFN- and not IL-2 by anergic human T cells correlates with assembly of an immature immune synapse Leo M. Carlin, Kumiko Yanagi, Adrienne Verhoef, Esther N. M. Nolte-'t Hoen, John Yates, Leanne Gardner, Jonathan Lamb, Giovanna Lombardi, Margaret J. Dallman, and Daniel M. Davis From the Division of Cell and Molecular Biology, South Kensington Campus, Imperial College, London; the Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College, London; the Department of Immunology, Hammersmith Campus, Imperial College, London, United Kingdom; and Translational Medicine and Genetics, Clinical Pharmacology and Discovery Medicine, GlaxoSmithKline, Greenford, Middlesex, United Kingdom. We report differences in the supramolecular organization of the immunologic synapse (IS) formed by resting and anergic human T cells with agonist peptide-loaded antigen-presenting cells (APCs). T cells reactive to influenza A hemagglutinin peptide or Fel d 1 peptide 4 were rendered both anergic and regulatory by incubation with high doses of agonist peptide in the absence of APCs. At the IS between resting T cells and peptide-loaded APCs, both CD3 and CD3 initially accumulate within a ring or arc before redistributing within 30 minutes to single or multiple foci more central to the contact. In contrast, at synapses formed by anergized T cells, CD3 and CD3 remained organized within an arc or ring and failed to redistribute centrally. However, intercellular communication between anergic human T cells and agonist peptide-loaded APCs was not a null event, since it triggered secretion of T-cell interferon (IFN-) but not, for example, interleukin 2 (IL-2). Thus, distinct organizations of CD3 at the T-cell IS correlate with different cytokine profiles; the mature IS formed by resting T cells correlates with their production of both IFN- and IL-2, whereas the immature IS formed by anergic T cells seems able to facilitate IFN- but not IL-2 production. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. L. Singleton, K. T. Roybal, Y. Sun, G. Fu, N. R. J. Gascoigne, N. S. C. van Oers, and C. Wulfing Spatiotemporal Patterning During T Cell Activation Is Highly Diverse Sci. Signal., April 7, 2009; 2(65): ra15 - ra15. [Abstract] [Full Text] [PDF] M. G. Marko, T. Ahmed, S. C. Bunnell, D. Wu, H. Chung, B. T. Huber, and S. N. Meydani Age-Associated Decline in Effective Immune Synapse Formation of CD4+ T Cells Is Reversed by Vitamin E Supplementation J. Immunol., February 1, 2007; 178(3): 1443 - 1449. [Abstract] [Full Text] [PDF] M. Zeyda, R. Geyeregger, M. Poglitsch, T. Weichhart, G. J. Zlabinger, S. Koyasu, W. H. Horl, T. M. Stulnig, B. Watschinger, and M. D. Saemann Impairment of T cell interactions with antigen-presenting cells by immunosuppressive drugs reveals involvement of calcineurin and NF-{kappa}B in immunological synapse formation J. Leukoc. Biol., January 1, 2007; 81(1): 319 - 327. [Abstract] [Full Text] [PDF]

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