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Blood, 15 December 2005, Vol. 106, No. 13, pp. 4086-4092. Prepublished online as a Blood First Edition Paper on August 30, 2005; DOI 10.1182/blood-2005-03-1072. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 2005-03-1072v1 106/13/4086    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Taussig, D. C. Articles by Bonnet, D. Search for Related Content PubMed PubMed Citation Articles by Taussig, D. C. Articles by Bonnet, D. Related Collections Hematopoiesis and Stem Cells Neoplasia Stem Cells in Hematology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Hematopoietic stem cells express multiple myeloid markers: implications for the origin and targeted therapy of acute myeloid leukemia David C. Taussig, Daniel J. Pearce, Catherine Simpson, Ama Z. Rohatiner, T. Andrew Lister, Gavin Kelly, Jennifer L. Luongo, Gwenn-aël H. Danet-Desnoyers, and Dominique Bonnet From the Haematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom; Division of Haematological Oncology, St Bartholomew's Hospital, West Smithfield, London, United Kingdom; Fluorescence Activated Cell Sorting Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom; Computational Genome Analysis Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom; and Department of Hematology/Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA. Human hematopoietic stem cells (HSCs) are generally regarded as being devoid of the markers expressed by differentiated blood cells, the lineage-specific antigens. However, recent work suggests that genes associated with the myeloid lineage are transcribed in mouse HSCs. Here, we explore whether myeloid genes are actually translated in human HSCs. We show that CD33, CD13, and CD123, well-established myeloid markers, are expressed on human long-term repopulating cells from cord blood and bone marrow. In addition, we demonstrate that nonobese diabetic/severe combined immunodeficiency (NOD/SCID) leukemia-initiating cells (SL-ICs) are restricted to the CD33+ fraction in 11 of 12 acute myeloid leukemia (AML) samples studied, indicating that leukemic stem cells (LSCs) express this antigen. This study changes our view of HSCs and the process of differentiation. Furthermore, based on the phenotypic similarity of HSCs and LSCs, our data provide support for the hypothesis that AML derives from an HSC. Our findings also provide a challenge to contemporary attempts to improve the outcome of AML using myeloid antigen-targeted therapies, given the potential for HSC killing. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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