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Blood, 15 July 2005, Vol. 106, No. 2, pp. 494-504. Prepublished online as a Blood First Edition Paper on March 22, 2005; DOI 10.1182/blood-2004-08-3280. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-08-3280v1 106/2/494    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Growney, J. D. Articles by Gilliland, D. G. Search for Related Content PubMed PubMed Citation Articles by Growney, J. D. Articles by Gilliland, D. G. Related Collections Gene Expression Hematopoiesis Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Loss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype Joseph D. Growney, Hirokazu Shigematsu, Zhe Li, Benjamin H. Lee, Jennifer Adelsperger, Rebecca Rowan, David P. Curley, Jeffery L. Kutok, Koichi Akashi, Ifor R. Williams, Nancy A. Speck, and D. Gary Gilliland From the Division of Hematology and the Department of Pathology, Brigham and Women's Hospital, Boston; Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA; Department of Biochemistry, Dartmouth Medical School, Hanover, NH; and Department of Pathology, Emory University, Atlanta, GA. Homozygous loss of function of Runx1 (Runt-related transcription factor 1 gene) during murine development results in an embryonic lethal phenotype characterized by a complete lack of definitive hematopoiesis. In light of recent reports of disparate requirements for hematopoietic transcription factors during development as opposed to adult hematopoiesis, we used a conditional gene-targeting strategy to effect the loss of Runx1 function in adult mice. In contrast with the critical role of Runx1 during development, Runx1 was not essential for hematopoiesis in the adult hematopoietic compartment, though a number of significant hematopoietic abnormalities were observed. Runx1 excision had lineage-specific effects on B- and T-cell maturation and pronounced inhibition of common lymphocyte progenitor production. Runx1 excision also resulted in inefficient platelet production. Of note, Runx1-deficient mice developed a mild myeloproliferative phenotype characterized by an increase in peripheral blood neutrophils, an increase in myeloid progenitor populations, and extramedullary hematopoiesis composed of maturing myeloid and erythroid elements. These findings indicate that Runx1 deficiency has markedly different consequences during development compared with adult hematopoiesis, and they provide insight into the phenotypic manifestations of Runx1 deficiency in hematopoietic malignancies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. R.A. Ehrhardt, A. Hijikata, H. Kitamura, O. Ohara, J.-Y. Wang, and M. D. Cooper Discriminating gene expression profiles of memory B cell subpopulations J. Exp. Med., July 14, 2008; (2008) jem.20072682. [Abstract] [Full Text] [PDF] S. D. Nimer Myelodysplastic syndromes Blood, May 15, 2008; 111(10): 4841 - 4851. [Abstract] [Full Text] [PDF] F. 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