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Blood, 15 July 2005, Vol. 106, No. 2, pp. 514-520. Prepublished online as a Blood First Edition Paper on April 5, 2005; DOI 10.1182/blood-2005-02-0598. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-02-0598v1 106/2/514    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abu-Daya, A. Articles by Guille, M. J. Search for Related Content PubMed PubMed Citation Articles by Abu-Daya, A. Articles by Guille, M. J. Related Collections Hematopoiesis and Stem Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Zygotic nucleosome assembly protein–like 1 has a specific, non–cell autonomous role in hematopoiesis Anita Abu-Daya, Wendy M. Steer, Alexandra F. Trollope, Christine E. Friedeberg, Roger K. Patient, Alan W. Thorne, and Matthew J. Guille From the School of Biological Sciences, Institute of Biomedical and Biomolecular Science, University of Portsmouth, Portsmouth, United Kingdom; and the Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom. Nucleosome assembly proteins (NAPs) bind core histones, facilitate chromatin remodeling, and can act as transcriptional coactivators. We previously described the isolation of a Xenopus NAP1-like (xNAP1L) cDNA, which encodes a member of this protein family. Its zygotic expression is restricted to neural cells, the outer cells of the ventral blood island (VBIs), and the ectoderm overlying the blood precursors. Here, we report that depletion of zygotic xNAP1L in embryos produces no obvious morphologic phenotype, but ablates -globin mRNA expression in the VBIs. Transcript levels of the hematopoietic precursor genes SCL and Xaml (Runx-1) are also reduced in the VBIs. SCL expression can be rescued by injection of xNAP1L mRNA into the ectoderm, showing that the effect of xNAP1L can be non–cell autonomous. Fli1 and Hex, genes expressed in hemangioblasts but subsequently endothelial markers, were unaffected, suggesting that xNAP1L is required for the hematopoietic lineage specifically. Our data are consistent with a requirement for xNAP1L upstream of SCL, and injection of SCL mRNA into xNAP1L-depleted embryos rescues -globin expression. Thus, xNAP1L, which belongs to a family of proteins previously believed to have general roles, has a specific function in hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. A. Horsfield, S. H. Anagnostou, J. K.-H. Hu, K. H. Y. Cho, R. Geisler, G. Lieschke, K. E. Crosier, and P. S. Crosier Cohesin-dependent regulation of Runx genes Development, July 15, 2007; 134(14): 2639 - 2649. [Abstract] [Full Text] [PDF] J. Zlatanova, C. Seebart, and M. Tomschik Nap1: taking a closer look at a juggler protein of extraordinary skills FASEB J, May 1, 2007; 21(7): 1294 - 1310. [Abstract] [Full Text] [PDF] A. Galichet and W. Gruissem Developmentally Controlled Farnesylation Modulates AtNAP1;1 Function in Cell Proliferation and Cell Expansion during Arabidopsis Leaf Development Plant Physiology, December 1, 2006; 142(4): 1412 - 1426. [Abstract] [Full Text] [PDF] C. Friedeberg, G. Scarlett, J. McGeeghan, A. Abu-daya, M. Guille, and G. Kneale Identification of a structural and functional domain in xNAP1 involved in protein-protein interactions Nucleic Acids Res., October 18, 2006; 34(17): 4893 - 4899. [Abstract] [Full Text] [PDF]

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