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Blood, 1 August 2005, Vol. 106, No. 3, pp. 1003-1007. Prepublished online as a Blood First Edition Paper on April 12, 2005; DOI 10.1182/blood-2004-12-4642. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-12-4642v1 106/3/1003    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Woolfson, A. Articles by Scott, M. A. Search for Related Content PubMed PubMed Citation Articles by Woolfson, A. Articles by Scott, M. A. Related Collections Immunobiology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Brief report Conservation of unique cell-surface CD antigen mosaics in HIV-1–infected individuals Adrian Woolfson, Justin Stebbing, Brian D. M. Tom, Kerryn J. Stoner, Walter R. Gilks, David P. Kreil, Stephen P. Mulligan, Larissa Belov, Jeremy S. Chrisp, Will Errington, Adrian Wildfire, Wendy N. Erber, Mark Bower, Brian Gazzard, Richard I. Christopherson, and Mike A. Scott From the University of Cambridge School of Clinical Medicine and the Department of Haematology, Addenbrooke's Hospital, Cambridge, United Kingdom; Chelsea and Westminster Hospital, London, United Kingdom; Medical Research Council Biostatistics Unit, Institute of Public Health, Cambridge, United Kingdom; University of Cambridge Department of Physics, Cavendish Laboratory, United Kingdom; and School of Molecular and Microbial Biosciences, University of Sydney, New South Wales, Australia. Cluster of differentiation (CD) antigens are expressed on cells of myeloid and lymphoid lineages. As most disease processes involve immune system activation or suppression, these antigens offer unique opportunities for monitoring host responses. Immunophenotyping using limited numbers of CD antigens enables differentiation states of immune system cells to be determined. Extended phenotyping involving parallel measurement of multiple CD antigens may help identify expression pattern signatures associated with specific disease states. To explore this possibility we have made a CD monoclonal antibody array and scanner, enabling the parallel immunophenotyping of leukocyte cell suspensions in a single and rapid analysis. To demonstrate this approach, we used the specific example of patients infected with human immunodeficiency virus type-1 (HIV-1). An invariant HIV-induced CD antigen signature has been defined that is both robust and independent of clinical outcome, composed of a unique profile of CD antigen expression levels that are both increased and decreased relative to internal controls. The results indicate that HIV-induced changes in CD antigen expression are disease specific and independent of outcome. Their invariant nature indicates an irreversible component to retroviral infection and suggests the utility of CD antigen expression patterns in other disease settings. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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