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 Blood, 15 August 2005, Vol. 106, No. 4, pp. 1215-1222.
Prepublished online as a Blood First Edition Paper on April 28, 2005; DOI 10.1182/blood-2004-12-4670.

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HEMATOPOIESIS

Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B henselae

Tanja Mändle, Hermann Einsele, Martin Schaller, Diana Neumann, Wichard Vogel, Ingo B. Autenrieth, and Volkhard A. J. Kempf

From the Institut für Medizinische Mikrobiologie und Hygiene; Medizinische Universitätsklinik II; and Universitäts-Hautklinik, Eberhard-Karls-Universität, Tübingen, Germany.

Although there is evidence that endothelial cells are important targets for human pathogenic Bartonella species, the primary niche of infection is unknown. Here we elucidated whether human CD34+ hematopoietic progenitor cells (HPCs) internalize B henselae and may serve as a potential niche of the pathogen. We showed that B henselae does not adhere to or invade human erythrocytes. In contrast, B henselae invades and persists in HPCs as shown by gentamicin protection assays, confocal laser scanning microscopy (CLSM), and electron microscopy (EM). Fluorescence-activated cell sorting (FACS) analysis of glycophorin A expression revealed that erythroid differentiation of HPCs was unaffected following infection with B henselae. The number of intracellular B henselae continuously increased over a 13-day period. When HPCs were infected with B henselae immediately after isolation, intracellular bacteria were subsequently detectable in differentiated erythroid cells on day 9 and day 13 after infection, as shown by CLSM, EM, and FACS analysis. Our data provide, for the first time, evidence that a bacterial pathogen is able to infect and persist in differentiating HPCs, and suggest that HPCs might serve as a potential primary niche in Bartonella infections.


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