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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1215-1222. Prepublished online as a Blood First Edition Paper on April 28, 2005; DOI 10.1182/blood-2004-12-4670. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-12-4670v1 106/4/1215    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mändle, T. Articles by Kempf, V. A. J. Search for Related Content PubMed PubMed Citation Articles by Mändle, T. Articles by Kempf, V. A. J. Related Collections Hematopoiesis and Stem Cells Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B henselae Tanja Mändle, Hermann Einsele, Martin Schaller, Diana Neumann, Wichard Vogel, Ingo B. Autenrieth, and Volkhard A. J. Kempf From the Institut für Medizinische Mikrobiologie und Hygiene; Medizinische Universitätsklinik II; and Universitäts-Hautklinik, Eberhard-Karls-Universität, Tübingen, Germany. Although there is evidence that endothelial cells are important targets for human pathogenic Bartonella species, the primary niche of infection is unknown. Here we elucidated whether human CD34+ hematopoietic progenitor cells (HPCs) internalize B henselae and may serve as a potential niche of the pathogen. We showed that B henselae does not adhere to or invade human erythrocytes. In contrast, B henselae invades and persists in HPCs as shown by gentamicin protection assays, confocal laser scanning microscopy (CLSM), and electron microscopy (EM). Fluorescence-activated cell sorting (FACS) analysis of glycophorin A expression revealed that erythroid differentiation of HPCs was unaffected following infection with B henselae. The number of intracellular B henselae continuously increased over a 13-day period. When HPCs were infected with B henselae immediately after isolation, intracellular bacteria were subsequently detectable in differentiated erythroid cells on day 9 and day 13 after infection, as shown by CLSM, EM, and FACS analysis. Our data provide, for the first time, evidence that a bacterial pathogen is able to infect and persist in differentiating HPCs, and suggest that HPCs might serve as a potential primary niche in Bartonella infections. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Salvatore, A. Casamassimi, L. Sommese, C. Fiorito, A. Ciccodicola, R. Rossiello, B. Avallone, V. Grimaldi, V. Costa, M. Rienzo, et al. Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells PNAS, July 8, 2008; 105(27): 9427 - 9432. [Abstract] [Full Text] [PDF] T. A. Florin, T. E. Zaoutis, and L. B. Zaoutis Beyond Cat Scratch Disease: Widening Spectrum of Bartonella henselae Infection Pediatrics, May 1, 2008; 121(5): e1413 - e1425. [Abstract] [Full Text] [PDF] R. Ridder-Schroter, A. Marx, M. Beer, D. Tappe, H.-W. Kreth, and H. J. Girschick Abscess-forming lymphadenopathy and osteomyelitis in children with Bartonella henselae infection J. Med. Microbiol., April 1, 2008; 57(4): 519 - 524. [Abstract] [Full Text] [PDF] J. Berghoff, J. Viezens, L. Guptill, M. Fabbi, and M. Arvand Bartonella henselae exists as a mosaic of different genetic variants in the infected host Microbiology, July 1, 2007; 153(7): 2045 - 2051. [Abstract] [Full Text] [PDF]

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