SEARCH:   Advanced

Blood, 15 August 2005, Vol. 106, No. 4, pp. 1432-1440. Prepublished online as a Blood First Edition Paper on May 5, 2005; DOI 10.1182/blood-2005-03-0944. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: 2005-03-0944v1 106/4/1432    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Condliffe, A. M. Articles by Hawkins, P. T. Search for Related Content PubMed PubMed Citation Articles by Condliffe, A. M. Articles by Hawkins, P. T. Related Collections Phagocytes Signal Transduction Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils Alison M. Condliffe, Keith Davidson, Karen E. Anderson, Chris D. Ellson, Tom Crabbe, Klaus Okkenhaug, Bart Vanhaesebroeck, Martin Turner, Louise Webb, Matthias P. Wymann, Emilio Hirsch, Thomas Ruckle, Montserrat Camps, Christian Rommel, Shaun P. Jackson, Edwin R. Chilvers, Len R. Stephens, and Phillip T. Hawkins From the Inositide Laboratory and Laboratory of Lymphocyte Signalling and Development, Babraham Institute, Cambridge, United Kingdom; Celltech R&D Ltd, Berkshire, United Kingdom; Ludwig Institute for Cancer Research, London, United Kingdom; Department of Biochemistry and Molecular Biology, London, United Kingdom; Centre of Biomedicine, Department of Clinical and Biological Science, University of Basel, Basel, Switzerland; Department of Genetics, Biology and Biochemistry, University of Torino, Torino, Italy; Serono Pharmaceutical Research Institute, Geneva, Switzerland; Australian Centre for Blood Diseases, Monash University, Victoria, Australia; and Respiratory Medicine Division, Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke's and Papworth Hospitals, Cambridge, United Kingdom. It is well established that preexposure of human neutrophils to proinflammatory cytokines markedly augments the production of reactive oxygen species (ROS) to subsequent stimuli. This priming event is thought to be critical for localizing ROS to the vicinity of the inflammation, maximizing their role in the resolution of the inflammation, and minimizing the damage to surrounding tissue. We have used a new generation of isoform-selective phosphoinositide 3-kinase (PI3K) inhibitors to show that ROS production under these circumstances is regulated by temporal control of class I PI3K activity. Stimulation of tumor necrosis factor- (TNF-)-primed human neutrophils with N-formyl-methionyl-leucyl-phenylalanine (fMLP) results in biphasic activation of PI3K; the first phase is largely dependent on PI3K, and the second phase is largely dependent on PI3K. The second phase of PI3K activation requires the first phase; it is this second phase that is augmented by TNF- priming and that regulates parallel activation of ROS production. Surprisingly, although TNF--primed mouse bone marrow-derived neutrophils exhibit superficially similar patterns of PI3K activation and ROS production in response to fMLP, these responses are substantially lower and largely dependent on PI3K alone. These results start to define which PI3K isoforms are responsible for modulating neutrophil responsiveness to infection and inflammation. (Blood. 2005; 106:1432-1440) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Bursting with PIP3 David A. Fruman Blood 2005 106: 1139-1140. [Full Text] [PDF] This article has been cited by other articles: I. Canobbio, L. Stefanini, L. Cipolla, E. Ciraolo, C. Gruppi, C. Balduini, E. Hirsch, and M. Torti Genetic evidence for a predominant role of PI3K{beta} catalytic activity in ITAM- and integrin-mediated signaling in platelets Blood, September 3, 2009; 114(10): 2193 - 2196. [Abstract] [Full Text] [PDF] C. Lecut, K. Frederix, D. M. Johnson, C. Deroanne, M. Thiry, C. Faccinetto, R. Maree, R. J. Evans, P. G. A. Volders, V. Bours, et al. P2X1 Ion Channels Promote Neutrophil Chemotaxis through Rho Kinase Activation J. Immunol., August 15, 2009; 183(4): 2801 - 2809. [Abstract] [Full Text] [PDF] E. Kingham and M. Welham Distinct roles for isoforms of the catalytic subunit of class-IA PI3K in the regulation of behaviour of murine embryonic stem cells J. Cell Sci., July 1, 2009; 122(13): 2311 - 2321. [Abstract] [Full Text] [PDF] R. D. Ye, F. Boulay, J. M. Wang, C. Dahlgren, C. Gerard, M. Parmentier, C. N. Serhan, and P. M. Murphy International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the Formyl Peptide Receptor (FPR) Family Pharmacol. Rev., June 1, 2009; 61(2): 119 - 161. [Abstract] [Full Text] [PDF] F. Morello, A. Perino, and E. Hirsch Phosphoinositide 3-kinase signalling in the vascular system Cardiovasc Res, May 1, 2009; 82(2): 261 - 271. [Abstract] [Full Text] [PDF] A. Saudemont, F. Garcon, H. Yadi, M. Roche-Molina, N. Kim, A. Segonds-Pichon, A. Martin-Fontecha, K. Okkenhaug, and F. Colucci p110{gamma} and p110{delta} isoforms of phosphoinositide 3-kinase differentially regulate natural killer cell migration in health and disease PNAS, April 7, 2009; 106(14): 5795 - 5800. [Abstract] [Full Text] [PDF] J. A. Marwick, G. Caramori, C. S. Stevenson, P. Casolari, E. Jazrawi, P. J. Barnes, K. Ito, I. M. Adcock, P. A. Kirkham, and A. Papi Inhibition of PI3K{delta} Restores Glucocorticoid Function in Smoking-induced Airway Inflammation in Mice Am. J. Respir. Crit. Care Med., April 1, 2009; 179(7): 542 - 548. [Abstract] [Full Text] [PDF] C. Billottet, L. Banerjee, B. Vanhaesebroeck, and A. Khwaja Inhibition of Class I Phosphoinositide 3-Kinase Activity Impairs Proliferation and Triggers Apoptosis in Acute Promyelocytic Leukemia without Affecting Atra-Induced Differentiation Cancer Res., February 1, 2009; 69(3): 1027 - 1036. [Abstract] [Full Text] [PDF] K. E. Anderson, K. B. Boyle, K. Davidson, T. A. M. Chessa, S. Kulkarni, G. E. Jarvis, A. Sindrilaru, K. Scharffetter-Kochanek, O. Rausch, L. R. Stephens, et al. CD18-dependent activation of the neutrophil NADPH oxidase during phagocytosis of Escherichia coli or Staphylococcus aureus is regulated by class III but not class I or II PI3Ks Blood, December 15, 2008; 112(13): 5202 - 5211. [Abstract] [Full Text] [PDF] A. S. Guerreiro, S. Fattet, B. Fischer, T. Shalaby, S. P. Jackson, S. M. Schoenwaelder, M. A. Grotzer, O. Delattre, and A. Arcaro Targeting the PI3K p110{alpha} Isoform Inhibits Medulloblastoma Proliferation, Chemoresistance, and Migration Clin. Cancer Res., November 1, 2008; 14(21): 6761 - 6769. [Abstract] [Full Text] [PDF] R. Heller, Q. Chang, G. Ehrlich, S. N. Hsieh, S. M. Schoenwaelder, P. J. Kuhlencordt, K. T. Preissner, E. Hirsch, and R. Wetzker Overlapping and distinct roles for PI3K{beta} and {gamma} isoforms in S1P-induced migration of human and mouse endothelial cells Cardiovasc Res, October 1, 2008; 80(1): 96 - 105. [Abstract] [Full Text] [PDF] A. S. Cowburn, A. M. Condliffe, N. Farahi, C. Summers, and E. R. Chilvers Advances in Neutrophil Biology: Clinical Implications Chest, September 1, 2008; 134(3): 606 - 612. [Abstract] [Full Text] [PDF] S. Kim, A. Garcia, S. P. Jackson, and S. P. Kunapuli Insulin-like growth factor-1 regulates platelet activation through PI3-K{alpha} isoform Blood, December 15, 2007; 110(13): 4206 - 4213. [Abstract] [Full Text] [PDF] N. Kim, A. Saudemont, L. Webb, M. Camps, T. Ruckle, E. Hirsch, M. Turner, and F. Colucci The p110delta catalytic isoform of PI3K is a key player in NK-cell development and cytokine secretion Blood, November 1, 2007; 110(9): 3202 - 3208. [Abstract] [Full Text] [PDF] S. M. Schoenwaelder, A. Ono, S. Sturgeon, S. M. Chan, P. Mangin, M. J. Maxwell, S. Turnbull, M. Mulchandani, K. Anderson, G. Kauffenstein, et al. Identification of a Unique Co-operative Phosphoinositide 3-Kinase Signaling Mechanism Regulating Integrin {alpha}IIbbeta3 Adhesive Function in Platelets J. Biol. Chem., September 28, 2007; 282(39): 28648 - 28658. [Abstract] [Full Text] [PDF] B. Kasper, E. Brandt, S. Brandau, and F. Petersen Platelet Factor 4 (CXC Chemokine Ligand 4) Differentially Regulates Respiratory Burst, Survival, and Cytokine Expression of Human Monocytes by Using Distinct Signaling Pathways J. Immunol., August 15, 2007; 179(4): 2584 - 2591. [Abstract] [Full Text] [PDF] C. D. Wrann, N. A. Tabriz, T. Barkhausen, A. Klos, M. van Griensven, H. C. Pape, D. O. Kendoff, R. Guo, P. A. Ward, C. Krettek, et al. The Phosphatidylinositol 3-Kinase Signaling Pathway Exerts Protective Effects during Sepsis by Controlling C5a-Mediated Activation of Innate Immune Functions J. Immunol., May 1, 2007; 178(9): 5940 - 5948. [Abstract] [Full Text] [PDF] U. A. Maus, M. Backi, C. Winter, M. Srivastava, M. K. Schwarz, T. Ruckle, J. C. Paton, D. Briles, M. Mack, T. Welte, et al. Importance of Phosphoinositide 3-Kinase {gamma} in the Host Defense against Pneumococcal Infection Am. J. Respir. Crit. Care Med., May 1, 2007; 175(9): 958 - 966. [Abstract] [Full Text] [PDF] K. K. Subramanian, Y. Jia, D. Zhu, B. T. Simms, H. Jo, H. Hattori, J. You, J. P. Mizgerd, and H. R. Luo Tumor suppressor PTEN is a physiologic suppressor of chemoattractant-mediated neutrophil functions Blood, May 1, 2007; 109(9): 4028 - 4037. [Abstract] [Full Text] [PDF] J. A. Deane, M. G. Kharas, J. S. Oak, L. N. Stiles, J. Luo, T. I. Moore, H. Ji, C. Rommel, L. C. Cantley, T. E. Lane, et al. T-cell function is partially maintained in the absence of class IA phosphoinositide 3-kinase signaling Blood, April 1, 2007; 109(7): 2894 - 2902. [Abstract] [Full Text] [PDF] H. Guillou, C. Lecureuil, K. E. Anderson, S. Suire, G. J. Ferguson, C. D. Ellson, A. Gray, N. Divecha, P. T. Hawkins, and L. R. Stephens Use of the GRP1 PH domain as a tool to measure the relative levels of PtdIns(3,4,5)P3 through a protein-lipid overlay approach J. Lipid Res., March 1, 2007; 48(3): 726 - 732. [Abstract] [Full Text] [PDF] D. M. McKay, J. L. Watson, A. Wang, J. Caldwell, D. Prescott, P. M. J. Ceponis, V. Di Leo, and J. Lu Phosphatidylinositol 3'-Kinase Is a Critical Mediator of Interferon-{gamma}-Induced Increases in Enteric Epithelial Permeability J. Pharmacol. Exp. Ther., March 1, 2007; 320(3): 1013 - 1022. [Abstract] [Full Text] [PDF] N. Molnarfi, L. Gruaz, J.-M. Dayer, and D. Burger Opposite Regulation of IL-1beta and Secreted IL-1 Receptor Antagonist Production by Phosphatidylinositide-3 Kinases in Human Monocytes Activated by Lipopolysaccharides or Contact with T Cells J. Immunol., January 1, 2007; 178(1): 446 - 454. [Abstract] [Full Text] [PDF] J. M. E. M. Cosemans, I. C. A. Munnix, R. Wetzker, R. Heller, S. P. Jackson, and J. W. M. Heemskerk Continuous signaling via PI3K isoforms beta and {gamma} is required for platelet ADP receptor function in dynamic thrombus stabilization Blood, November 1, 2006; 108(9): 3045 - 3052. [Abstract] [Full Text] [PDF] C. D. Ellson, K. Davidson, G. J. Ferguson, R. O'Connor, L. R. Stephens, and P. T. Hawkins Neutrophils from p40phox-/- mice exhibit severe defects in NADPH oxidase regulation and oxidant-dependent bacterial killing J. Exp. Med., August 7, 2006; 203(8): 1927 - 1937. [Abstract] [Full Text] [PDF] I. Boulven, S. Levasseur, S. Marois, G. Pare, E. Rollet-Labelle, and P. H. Naccache Class IA Phosphatidylinositide 3-Kinases, rather than p110{gamma}, Regulate Formyl-Methionyl-Leucyl-Phenylalanine-Stimulated Chemotaxis and Superoxide Production in Differentiated Neutrophil-Like PLB-985 Cells. J. Immunol., June 15, 2006; 176(12): 7621 - 7627. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020