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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1488-1494. Prepublished online as a Blood First Edition Paper on May 3, 2005; DOI 10.1182/blood-2005-01-0264. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-01-0264v1 106/4/1488    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abedi, M. Articles by Quesenberry, P. J. Search for Related Content PubMed PubMed Citation Articles by Abedi, M. Articles by Quesenberry, P. J. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Critical variables in the conversion of marrow cells to skeletal muscle Mehrdad Abedi, Deborah A. Greer, Bethany M. Foster, Gerald A. Colvin, Joshua A. Harpel, Delia A. Demers, Jeffery Pimentel, Mark S. Dooner, and Peter J. Quesenberry From the Roger Williams Medical Center, Department of Research, Providence, RI. We have studied conversion of marrow cells to skeletal muscle in cardiotoxin-injured anterior tibialis muscle in a green fluorescent protein (GFP) to C57BL/6 transplantation model and ascertained that total body irradiation (TBI) with establishment of chimerism is a critical factor. Local irradiation has little effect in lower doses and was detrimental at higher doses. Whole body (1000 cGy) with shielding of the leg or a combination of 500 cGy TBI and 500 cGy local radiations was found to give the best results. In non-obese diabetic-severe combined immunodeficient (NOD-SCID) recipients, we were able to show that conversion could occur without radiation, albeit at relatively lower levels. Within 3 days of cardiotoxin injury, GFP-positive mononuclear cells were seen in the muscle, and within 2 weeks GFP-positive muscle fibers were identified. Conversion rates were increased by increasing donor-cell dose. Timing of the cardiotoxin injury relative to the transplantation was critical. These studies show that variables in transplantation and injury are critical features of marrow-to-muscle conversions. Irradiation primarily effects conversion by promoting chimerism. These data may explain the differences in the literature for the frequency of marrow-to-skeletal muscle conversion and can set a platform for future models and perhaps clinical protocols. (Blood. 2005;106:1488-1494) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Magrassi, P. Grimaldi, A. Ibatici, M. Corselli, L. Ciardelli, S. Castello, M. Podesta, F. Frassoni, and F. Rossi Induction and Survival of Binucleated Purkinje Neurons by Selective Damage and Aging J. Neurosci., September 12, 2007; 27(37): 9885 - 9892. [Abstract] [Full Text] [PDF] J. M. Aliotta, F. M. Sanchez-Guijo, G. J. Dooner, K. W. Johnson, M. S. Dooner, K. A. Greer, D. Greer, J. Pimentel, L. M. Kolankiewicz, N. Puente, et al. Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation Stem Cells, September 1, 2007; 25(9): 2245 - 2256. [Abstract] [Full Text] [PDF] N. Salah-Mohellibi, G. Millet, I. Andre-Schmutz, B. Desforges, R. Olaso, N. Roblot, S. Courageot, G. Bensimon, M. Cavazzana-Calvo, and J. Melki Bone Marrow Transplantation Attenuates the Myopathic Phenotype of a Muscular Mouse Model of Spinal Muscular Atrophy Stem Cells, December 1, 2006; 24(12): 2723 - 2732. [Abstract] [Full Text] [PDF]

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