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Blood, 1 September 2005, Vol. 106, No. 5, pp. 1574-1580. Prepublished online as a Blood First Edition Paper on May 3, 2005; DOI 10.1182/blood-2005-03-1017. This Article Full Text Full Text (PDF) Supplemental Table and Figure All Versions of this Article: 2005-03-1017v1 106/5/1574    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kotton, D. N. Articles by Mulligan, R. C. Search for Related Content PubMed PubMed Citation Articles by Kotton, D. N. Articles by Mulligan, R. C. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity Darrell N. Kotton, Attila J. Fabian, and Richard C. Mulligan From the Department of Genetics, Harvard Medical School and the Division of Molecular Medicine, Children's Hospital, Boston, MA; and the Section of Pulmonary, Allergy, and Critical Care, Department of Medicine, Boston University School of Medicine. A number of recent reports have documented that cells possessing hematopoietic-reconstitution ability can be identified and isolated from a variety of solid organs in the adult animal. In all studies to date, however, purified organ-derived stem cells demonstrate a diminished repopulating capacity relative to that of purified bone marrow–derived hematopoietic stem cells (BM HSCs). It has therefore been unclear whether organ-derived HSCs possess functional properties distinct from those of BM HSCs, or simply have not been purified to a comparable extent. Here we report the identification of a rare subset of cells in adult murine liver that possess potent blood-repopulating potential, approaching that of BM HSCs. The cells, isolated on the basis of dye-efflux activity and CD45 expression (termed CD45+ liver side population [SP] tip cells), exhibit a surface phenotype similar to that of freshly isolated BM HSCs derived from normal adult animals, but are phenotypically distinct in that they do not express the stem-cell marker c-kit. Single-cell transplantation studies indicate that CD45+ liver SP tip cells can be generated from BM HSCs, suggesting a relationship between stem-cell populations in the liver and bone marrow compartments. Overall, these studies have important implications for understanding extramedullary hematopoiesis, and may be relevant to current strategies aimed at inducing tolerance to transplanted organs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A. Wilson, L. W. Kwok, A.-H. Hovav, S. J. Ohle, F. F. Little, A. Fine, and D. N. Kotton Sustained Expression of {alpha}1-Antitrypsin after Transplantation of Manipulated Hematopoietic Stem Cells Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 133 - 141. [Abstract] [Full Text] [PDF] S. Khurana and A. Mukhopadhyay Characterization of the Potential Subpopulation of Bone Marrow Cells Involved in the Repair of Injured Liver Tissue Stem Cells, June 1, 2007; 25(6): 1439 - 1447. [Abstract] [Full Text] [PDF] M. Drouet, J.-F. Mayol, F. Norol, A. Peinnequin, J.-P. Zarski, C. Letoublon, and F. Herodin Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys Haematologica, February 1, 2007; 92(2): 248 - 251. [Abstract] [Full Text] [PDF] D. N. Kotton, A. J. Fabian, and R. C. Mulligan Failure of Bone Marrow to Reconstitute Lung Epithelium Am. J. Respir. Cell Mol. Biol., October 1, 2005; 33(4): 328 - 334. [Abstract] [Full Text] [PDF]

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