|
|
Blood, 1 September 2005, Vol. 106, No. 5, pp. 1601-1603.
Prepublished online as a Blood First Edition Paper on May 24, 2005; DOI 10.1182/blood-2005-03-0987.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
Brief report
Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation
Elizabeth S. Ng,
Richard P. Davis,
Lisa Azzola,
Edouard G. Stanley, and
Andrew G. Elefanty
From the Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
To realize the therapeutic potential of human embryonic stem cells (hESCs), it is necessary to regulate their differentiation in a uniform and reproducible manner. We have developed a method in which known numbers of hESCs in serum-free medium were aggregated by centrifugation to foster the formation of embryoid bodies (EBs) of uniform size (spin EBs). These spin EBs differentiated efficiently and synchronously, as evidenced by the sequential expression of molecular markers representing stem cells, primitive streak, and mesoderm. In the presence of hematopoietic growth factors, reproducible differentiation was achieved with blood cells formed in more than 90% of EBs. Using chimeric EBs generated from mixtures of green fluorescence protein–positive (GFP+) and GFP– hESCs in a clonogenic assay, hematopoietic precursor frequency was estimated to be approximately 1:500 input cells. This method of EB formation provides a generally applicable means for modulating and objectively monitoring the directed differentiation of hESCs.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
A new spin on hESC differentiation
- Manfred Baetscher
Blood 2005 106: 1509.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
C. Qiu, E. N. Olivier, M. Velho, and E. E. Bouhassira
Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells
Blood,
February 15, 2008;
111(4):
2400 - 2408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. P. Davis, E. S. Ng, M. Costa, A. K. Mossman, K. Sourris, A. G. Elefanty, and E. G. Stanley
Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors
Blood,
February 15, 2008;
111(4):
1876 - 1884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pick, L. Azzola, A. Mossman, E. G. Stanley, and A. G. Elefanty
Differentiation of Human Embryonic Stem Cells in Serum-Free Medium Reveals Distinct Roles for Bone Morphogenetic Protein 4, Vascular Endothelial Growth Factor, Stem Cell Factor, and Fibroblast Growth Factor 2 in Hematopoiesis
Stem Cells,
September 1, 2007;
25(9):
2206 - 2214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. Carpenedo, C. Y. Sargent, and T. C. McDevitt
Rotary Suspension Culture Enhances the Efficiency, Yield, and Homogeneity of Embryoid Body Differentiation
Stem Cells,
September 1, 2007;
25(9):
2224 - 2234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Burridge, D. Anderson, H. Priddle, M. D. Barbadillo Munoz, S. Chamberlain, C. Allegrucci, L. E. Young, and C. Denning
Improved Human Embryonic Stem Cell Embryoid Body Homogeneity and Cardiomyocyte Differentiation from a Novel V-96 Plate Aggregation System Highlights Interline Variability
Stem Cells,
April 1, 2007;
25(4):
929 - 938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bhatia
Hematopoietic Development from Human Embryonic Stem Cells
Hematology,
January 1, 2007;
2007(1):
11 - 16.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Vodyanik, J. A. Thomson, and I. I. Slukvin
Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures
Blood,
September 15, 2006;
108(6):
2095 - 2105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-H. Chang, A. M. Nelson, H. Cao, L. Wang, B. Nakamoto, C. B. Ware, and T. Papayannopoulou
Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin
Blood,
September 1, 2006;
108(5):
1515 - 1523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Trounson
The Production and Directed Differentiation of Human Embryonic Stem Cells
Endocr. Rev.,
April 1, 2006;
27(2):
208 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Lensch and G. Q. Daley
Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells
Blood,
April 1, 2006;
107(7):
2605 - 2612.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
