|
|
Blood, 1 September 2005, Vol. 106, No. 5, pp. 1612-1620.
Prepublished online as a Blood First Edition Paper on May 17, 2005; DOI 10.1182/blood-2004-08-3367.
 Previous Article | Table of Contents | Next Article 
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Mouse Polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF1 expression
Yuko Katoh-Fukui,
Akiko Owaki,
Yoshiro Toyama,
Masatomo Kusaka,
Yuko Shinohara,
Mamiko Maekawa,
Kiyotaka Toshimori, and
Ken-ichirou Morohashi
From the Division for Sex Differentiation, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan; the Department of Molecular Biomechanism, School of Life Sciences, Graduate University for Advanced Studies, Okazaki, Japan; the Department of Anatomy, Graduate School of Medicine, Chiba University, Chiba, Japan; and the Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi, Japan.
Mice with disrupted mammalian PcG (Polycomb group) genes commonly show skeletal transformation of anterior-posterior identities. Disruption of the murine M33 gene, a PcG member, displayed posterior transformation of the vertebral columns and sternal ribs. In addition, failure of T-cell expansion and hypoplasia and sex-reversal of the gonads, have been observed. In the present study, we identified defects in the splenic and adrenal formation of M33–knock-out (KO) mice on a C57BL/6 genetic background. The spleen in these animals was smaller than in the wild-type mice and was spotted red because of nonuniform distribution of blood cells. Histologic examination revealed disorganization of the vascular endothelium and its surrounding structures, and immunohistochemistry demonstrated disturbances in vascular formation and colonization of immature hematopoietic cells. These splenic phenotypes observed in the M33-KO mice were quite similar to those seen in Ad4BP/SF1 (Nr5a1) knock-outs. Moreover, the adrenal glands of M33-KO and Ad4BP/SF1 heterozygous KO mice were smaller than those of the wild-type mice. Western blot, immunohistochemistry, and reverse transcriptase–polymerase chain reaction (RT-PCR) analyses of the M33 knock-outs all indicated significantly low expression of adrenal 4 binding protein/steroidogenic factor-1 (Ad4BP/SF-1), indicating that M33 is an essential upstream regulator of Ad4BP/SF1. In agreement with these observations, chromatin immunoprecipitation assays with adrenocortical Y-1 cells revealed direct binding of the M33-containing PcG to the Ad4BP/SF1 gene locus.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Doghman, T. Karpova, G. A. Rodrigues, M. Arhatte, J. De Moura, L. R. Cavalli, V. Virolle, P. Barbry, G. P. Zambetti, B. C. Figueiredo, et al.
Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer
Mol. Endocrinol.,
December 1, 2007;
21(12):
2968 - 2987.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Val, J.-P. Martinez-Barbera, and A. Swain
Adrenal development is initiated by Cited2 and Wt1 through modulation of Sf-1 dosage
Development,
June 15, 2007;
134(12):
2349 - 2358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wilhelm, S. Palmer, and P. Koopman
Sex Determination and Gonadal Development in Mammals
Physiol Rev,
January 1, 2007;
87(1):
1 - 28.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zubair, S. Ishihara, S. Oka, K. Okumura, and K.-i. Morohashi
Two-Step Regulation of Ad4BP/SF-1 Gene Transcription during Fetal Adrenal Development: Initiation by a Hox-Pbx1-Prep1 Complex and Maintenance via Autoregulation by Ad4BP/SF-1.
Mol. Cell. Biol.,
June 1, 2006;
26(11):
4111 - 4121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Shima, M. Zubair, S. Ishihara, Y. Shinohara, S. Oka, S. Kimura, S. Okamoto, Y. Minokoshi, S. Suita, and K.-i. Morohashi
Ventromedial Hypothalamic Nucleus-Specific Enhancer of Ad4BP/SF-1 Gene
Mol. Endocrinol.,
November 1, 2005;
19(11):
2812 - 2823.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
