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Blood, 1 September 2005, Vol. 106, No. 5, pp. 1718-1725. Prepublished online as a Blood First Edition Paper on May 17, 2005; DOI 10.1182/blood-2004-12-4762. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-12-4762v1 106/5/1718    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mavilio, D. Articles by Fauci, A. S. Search for Related Content PubMed PubMed Citation Articles by Mavilio, D. Articles by Fauci, A. S. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Identification of NKG2A and NKp80 as specific natural killer cell markers in rhesus and pigtailed monkeys Domenico Mavilio, Janet Benjamin, Diana Kim, Gabriella Lombardo, Marybeth Daucher, Audrey Kinter, Elizabeth Nies-Kraske, Emanuela Marcenaro, Alessandro Moretta, and Anthony S. Fauci From the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD; the Dipartimento di Medicina Sperimentale, University of Genoa; and the Centro di Eccellenza per la Ricerca Biomedica, Genoa, Italy. Investigations of natural killer (NK) cells in simian models of disease have been hampered by a lack of appropriate phenotypic markers and by an inadequate understanding of the regulation of NK cell activities. In the present study, a panel of monoclonal antibodies (mAbs) specific for various human NK receptors was screened for cross-reactivity with NK cells from rhesus macaques and pigtailed macaques. Flow cytometric analyses using anti-human NKG2A and anti-human NKp80 mAbs individually, and particularly in combination with anti-CD16 mAb, allowed for the identification of the entire NK cell population in both species. NK cells in monkeys were generally identified by negative selection of peripheral blood mononuclear cells (PBMCs) for the absence of T-cell, B-cell, and monocyte markers. mAb-mediated ligation of NKp80 induced NK cell cytotoxicity, while in the case of NKG2A it displayed a clear capability to inhibit the lysis of target cells by NK cells from macaques, as well as from humans. This new phenotypic and functional characterization of NKG2A and NKp80 in rhesus and pigtailed macaque NK cells provides a new approach in the analysis of their innate immune system. (Blood. 2005;106:1718-1725) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. N. Brown, A. Trichel, and S. M. Barratt-Boyes Parallel Loss of Myeloid and Plasmacytoid Dendritic Cells from Blood and Lymphoid Tissue in Simian AIDS J. Immunol., June 1, 2007; 178(11): 6958 - 6967. [Abstract] [Full Text] [PDF] Q. Wei, J. W. Stallworth, P. J. Vance, J. A. Hoxie, and P. N. Fultz Simian Immunodeficiency Virus (SIV)/Immunoglobulin G Immune Complexes in SIV-Infected Macaques Block Detection of CD16 but Not Cytolytic Activity of Natural Killer Cells. Clin. Vaccine Immunol., July 1, 2006; 13(7): 768 - 778. [Abstract] [Full Text] [PDF]

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