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Blood, 15 September 2005, Vol. 106, No. 6, pp. 2083-2090.
Prepublished online as a Blood First Edition Paper on June 2, 2005; DOI 10.1182/blood-2005-01-0283.
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IMMUNOBIOLOGY
The development of functional B lymphocytes in conditional PU.1 knock-out mice
Matthew Polli,
Aleksandar Dakic,
Amanda Light,
Li Wu,
David M. Tarlinton, and
Stephen L. Nutt
From The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interleukin-4 (IL-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage. (Blood. 2005;106:2083-2090)
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