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 Blood, 1 October 2005, Vol. 106, No. 7, pp. 2235-2243.
Prepublished online as a Blood First Edition Paper on June 9, 2005; DOI 10.1182/blood-2004-11-4547.

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PLENARY PAPERS

B-cell depletion inhibits arthritis in a collagen-induced arthritis (CIA) model, but does not adversely affect humoral responses in a respiratory syncytial virus (RSV) vaccination model

Kyri Dunussi-Joannopoulos, Gerald E. Hancock, Arthur Kunz, Martin Hegen, Xiaochuan X. Zhou, Barbara J. Sheppard, Jennifer Lamothe, Evelyn Li, Hak-Ling Ma, Philip R. Hamann, Nitin K. Damle, and Mary Collins

From the Departments of Inflammation, Wyeth Vaccines, Chemical & Screening Sciences, Investigative Pathology, and Oncology Discovery, Wyeth Research, Cambridge, MA

We report the development of a mouse B cell-depleting immunoconjugate (anti-CD22 monoclonal antibody [mAb] conjugated to calicheamicin) and its in vivo use to characterize the kinetics of CD22+ B-cell depletion and reconstitution in murine primary and secondary lymphoid tissues. The effect of B-cell depletion was further studied in a murine collagen-induced arthritis (CIA) model and a respiratory syncytial virus (RSV) vaccination model. Our results show that (1) the immunoconjugate has B-cell-specific in vitro and in vivo cytotoxicity; (2) B-cell reconstitution starts in the bone marrow and spleen around day 30 after depletion and is completed in all tissues tested by day 50; (3) B-cell depletion inhibits the development of clinical and histologic arthritis in the CIA model; (4) depletion of type II collagen antibody levels is not necessary for clinical and histologic prevention of CIA; and (5) B-cell depletion does not adversely affect memory antibody responses after challenge nor clearance of infectious virus from lungs in the RSV vaccination model. These results demonstrate for the first time that only B-cell reduction but not type II collagen antibody levels correlate with the prevention of arthritis and represent key insights into the role of CD22-targeted B-cell depletion in mouse autoimmunity and vaccination models.


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