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Blood, 1 October 2005, Vol. 106, No. 7, pp. 2318-2328. Prepublished online as a Blood First Edition Paper on June 16, 2005; DOI 10.1182/blood-2005-02-0557. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 2005-02-0557v1 106/7/2318    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Reynaud, D. Articles by Pflumio, F. Search for Related Content PubMed PubMed Citation Articles by Reynaud, D. Articles by Pflumio, F. Related Collections Hematopoiesis and Stem Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment Damien Reynaud, Emmanuel Ravet, Monique Titeux, Frédéric Mazurier, Laurent Rénia, Anne Dubart-Kupperschmitt, Paul-Henri Roméo, and Françoise Pflumio From the Department of Hematology, Institut Cochin, U567 Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS) UMR 8104, Paris, France; INSERM E0217, Université V Segalen, Bordeaux, France; and the Department of Immunology, Institut Cochin, INSERM U567, Paris, France. The fate of hematopoietic stem cells (HSCs) is regulated through a combinatorial action of proteins that determine their self-renewal and/or their commitment to differentiation. Stem cell leukemia/T-cell acute lymphoblastic leukemia 1 (SCL/TAL1), a basic helix-loop-helix (bHLH) transcription factor, plays key roles in controlling the development of primitive and definitive hematopoiesis during mouse development but its function in adult HSCs is still a matter of debate. We report here that the lentiviral-mediated enforced expression of TAL1 in human CD34+ cells marginally affects in vitro the differentiation of committed progenitors, whereas in vivo the repopulation capacity of the long-term SCID (severe combined immunodeficient) mouse–repopulating cells (LT-SRCs) is enhanced. As a consequence, the production of SRC-derived multipotent progenitors as well as erythroid- and myeloid-differentiated cells is increased. Looking at the lymphoid compartment, constitutive TAL1-enforced expression impairs B- but not T-cell differentiation. Expression of a mutant TAL1 protein that cannot bind DNA specifically impairs human LT-SRC amplification, indicating a DNA-binding dependent effect of TAL1 on primitive cell populations. These results indicate that TAL1 expression level regulates immature human hematopoietic cell self-renewal and that this regulation requires TAL1 DNA-binding activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Brunet de la Grange, E. Zink, F. Armstrong, M.-C. Rouyez, and F. Pflumio Impairment of Granulo-Monocytic Development of Human Common Myeloid Progenitors but Not of Granulo-Monocytic Progenitors by Decreasing Stem Cell Leukemia/T-Cell Acute Leukemia 1 Expression Stem Cells, June 1, 2008; 26(6): 1658 - 1662. [Abstract] [Full Text] [PDF] J.-R. Landry, S. Kinston, K. Knezevic, M. F.T.R. de Bruijn, N. Wilson, W. T. Nottingham, M. Peitz, F. Edenhofer, J. E. Pimanda, K. Ottersbach, et al. Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver Blood, March 15, 2008; 111(6): 3005 - 3014. [Abstract] [Full Text] [PDF] L. Nie, H. Wu, and X.-H. Sun Ubiquitination and Degradation of Tal1/SCL Are Induced by Notch Signaling and Depend on Skp2 and CHIP J. Biol. Chem., January 11, 2008; 283(2): 684 - 692. [Abstract] [Full Text] [PDF] G. M. Crooks Another piece for the SCL/TAL1 puzzle Blood, November 1, 2006; 108(9): 2886 - 2886. [Full Text] [PDF] P. B. de la Grange, F. Armstrong, V. Duval, M.-C. Rouyez, N. Goardon, P.-H. Romeo, and F. Pflumio Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells Blood, November 1, 2006; 108(9): 2998 - 3004. [Abstract] [Full Text] [PDF] C. Capron, Y. Lecluse, A. L. Kaushik, A. Foudi, C. Lacout, D. Sekkai, I. Godin, O. Albagli, I. Poullion, F. Svinartchouk, et al. The SCL relative LYL-1 is required for fetal and adult hematopoietic stem cell function and B-cell differentiation Blood, June 15, 2006; 107(12): 4678 - 4686. [Abstract] [Full Text] [PDF] Z.-j. Ye, Y. Kluger, Z. Lian, and S. M. Weissman Two types of precursor cells in a multipotential hematopoietic cell line PNAS, December 20, 2005; 102(51): 18461 - 18466. [Abstract] [Full Text] [PDF]

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