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Blood, 1 October 2005, Vol. 106, No. 7, pp. 2366-2374. Prepublished online as a Blood First Edition Paper on April 28, 2005; DOI 10.1182/blood-2004-10-4166. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-10-4166v1 106/7/2366    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Law, H. K. W. Articles by Lau, Y. L. Search for Related Content PubMed PubMed Citation Articles by Law, H. K. W. Articles by Lau, Y. L. Related Collections Immunobiology Phagocytes Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Chemokine up-regulation in SARS-coronavirus–infected, monocyte-derived human dendritic cells Helen K. W. Law, Chung Yan Cheung, Hoi Yee Ng, Sin Fun Sia, Yuk On Chan, Winsie Luk, John M. Nicholls, J. S. Malik Peiris, and Yu Lung Lau From the Department of Paediatrics and Adolescent Medicine, the Department of Microbiology, and the Department of Pathology, Hong Kong Jockey Club Clinical Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China. Lymphopenia and increasing viral load in the first 10 days of severe acute respiratory syndrome (SARS) suggested immune evasion by SARS-coronavirus (CoV). In this study, we focused on dendritic cells (DCs) which play important roles in linking the innate and adaptive immunity. SARS-CoV was shown to infect both immature and mature human monocyte-derived DCs by electron microscopy and immunofluorescence. The detection of negative strands of SARS-CoV RNA in DCs suggested viral replication. However, no increase in viral RNA was observed. Using cytopathic assays, no increase in virus titer was detected in infected DCs and cell-culture supernatant, confirming that virus replication was incomplete. No induction of apoptosis or maturation was detected in SARS-CoV–infected DCs. The SARS-CoV–infected DCs showed low expression of antiviral cytokines (interferon [IFN-], IFN-, IFN-, and interleukin 12p40 [IL-12p40]), moderate up-regulation of proinflammatory cytokines (tumor necrosis factor [TNF-] and IL-6) but significant up-regulation of inflammatory chemokines (macrophage inflammatory protein 1 [MIP-1], regulated on activation normal T cell expressed and secreted [RANTES]), interferon-inducible protein of 10 kDa [IP-10], and monocyte chemoattractant protein 1 [MCP-1]). The lack of antiviral cytokine response against a background of intense chemokine up-regulation could represent a mechanism of immune evasion by SARS-CoV. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Netland, D. K. Meyerholz, S. Moore, M. Cassell, and S. Perlman Severe Acute Respiratory Syndrome Coronavirus Infection Causes Neuronal Death in the Absence of Encephalitis in Mice Transgenic for Human ACE2 J. 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Cheung, H. C. H. Yim, and A. S. Y. Lau Role for Nonstructural Protein 1 of Severe Acute Respiratory Syndrome Coronavirus in Chemokine Dysregulation J. Virol., January 1, 2007; 81(1): 416 - 422. [Abstract] [Full Text] [PDF] C.-P. Chan, K.-L. Siu, K.-T. Chin, K.-Y. Yuen, B. Zheng, and D.-Y. Jin Modulation of the unfolded protein response by the severe acute respiratory syndrome coronavirus spike protein. J. Virol., September 1, 2006; 80(18): 9279 - 9287. [Abstract] [Full Text] [PDF] M. Spiegel, K. Schneider, F. Weber, M. Weidmann, and F. T. Hufert Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells J. Gen. Virol., July 1, 2006; 87(7): 1953 - 1960. [Abstract] [Full Text] [PDF] S. R. Weiss and S. Navas-Martin Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 635 - 664. [Abstract] [Full Text] [PDF]

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