SEARCH:   Advanced

Blood, 15 October 2005, Vol. 106, No. 8, pp. 2818-2826. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2004-11-4321. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: 2004-11-4321v1 106/8/2818    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rimoldi, M. Articles by Rescigno, M. Search for Related Content PubMed PubMed Citation Articles by Rimoldi, M. Articles by Rescigno, M. Related Collections Immunobiology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Monocyte-derived dendritic cells activated by bacteria or by bacteria-stimulated epithelial cells are functionally different Monica Rimoldi, Marcello Chieppa, Paola Larghi, Marisa Vulcano, Paola Allavena, and Maria Rescigno From the Department of Experimental Oncology, European Institute of Oncology; Department of Immunology, Istituto di Ricerche Farmacologiche "Mario Negri," Milan, Italy; and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD. Dendritic cells (DCs) are able to open the tight junctions between adjacent epithelial cells (ECs) and to take up both invasive and noninvasive bacteria directly from the intestinal lumen. In this study, we describe a tight cross talk between ECs and human monocyte-derived DCs (MoDCs) in bacterial handling across epithelial monolayers. We show that the release of proinflammatory mediators by ECs in response to bacteria is dependent on bacterial invasiveness and on the presence of flagella. This correlates with the capacity of EC-derived factors to modulate MoDC function. MoDCs incubated with supernatants of bacteria-treated ECs are "noninflammatory" as they release interleukin-10 (IL-10) but not IL-12 and can drive only T helper (Th)-2 type T cells. Moreover, noninflammatory MoDCs release chemokines aimed at recruiting Th2 and T-regulatory cells. In contrast, when MoDCs are incubated with ECs and bacteria in a transwell coculture system, and can contact directly the bacteria across stimulated EC monolayers, they are more inflammatory as they release IL-12 and IL-10 and induce both Th1 and Th2 responses. These results suggest that ECs are not simply a barrier to bacteria entering via the oral route, but they actively influence the activating properties of DCs. (Blood. 2005;106:2818-2826) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: V. Cerovic, C. D. Jenkins, A. G. C. Barnes, S. W. F. Milling, G. G. MacPherson, and L. S. Klavinskis Hyporesponsiveness of Intestinal Dendritic Cells to TLR Stimulation Is Limited to TLR4 J. Immunol., February 15, 2009; 182(4): 2405 - 2415. [Abstract] [Full Text] [PDF] G. Rotta, G. Matteoli, E. Mazzini, P. Nuciforo, M. P. Colombo, and M. Rescigno Contrasting roles of SPARC-related granuloma in bacterial containment and in the induction of anti-Salmonella typhimurium immunity J. Exp. Med., March 17, 2008; 205(3): 657 - 667. [Abstract] [Full Text] [PDF] I. D. Iliev, G. Matteoli, and M. Rescigno The yin and yang of intestinal epithelial cells in controlling dendritic cell function J. Exp. Med., October 1, 2007; 204(10): 2253 - 2257. [Abstract] [Full Text] [PDF] M. A. Nolte, S. LeibundGut-Landmann, O. Joffre, and C. R. e Sousa Dendritic cell quiescence during systemic inflammation driven by LPS stimulation of radioresistant cells in vivo J. Exp. Med., June 11, 2007; 204(6): 1487 - 1501. [Abstract] [Full Text] [PDF] M. Palazzo, A. Balsari, A. Rossini, S. Selleri, C. Calcaterra, S. Gariboldi, L. Zanobbio, F. Arnaboldi, Y. F. Shirai, G. Serrao, et al. Activation of Enteroendocrine Cells via TLRs Induces Hormone, Chemokine, and Defensin Secretion J. Immunol., April 1, 2007; 178(7): 4296 - 4303. [Abstract] [Full Text] [PDF] C. L. Butts, S. A. Shukair, K. M. Duncan, E. Bowers, C. Horn, E. Belyavskaya, L. Tonelli, and E. M. Sternberg Progesterone inhibits mature rat dendritic cells in a receptor-mediated fashion Int. Immunol., March 1, 2007; 19(3): 287 - 296. [Abstract] [Full Text] [PDF] M. Chieppa, M. Rescigno, A. Y.C. Huang, and R. N. Germain Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement J. Exp. Med., December 25, 2006; 203(13): 2841 - 2852. [Abstract] [Full Text] [PDF] A. Ciabattini, A. M. Cuppone, R. Pulimeno, F. Iannelli, G. Pozzi, and D. Medaglini Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii. Clin. Vaccine Immunol., September 1, 2006; 13(9): 1037 - 1043. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020