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Blood, 1 November 2005, Vol. 106, No. 9, pp. 3012-3019. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2005-01-0433. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-01-0433v1 106/9/3012    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Baksh, D. Articles by Zandstra, P. W. Search for Related Content PubMed PubMed Citation Articles by Baksh, D. Articles by Zandstra, P. W. Related Collections Hematopoiesis and Stem Cells Stem Cells in Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Soluble factor cross-talk between human bone marrow-derived hematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor cell compartment Dolores Baksh, John E. Davies, and Peter W. Zandstra From the Institute of Biomaterials and Biomedical Engineering, Department of Chemical Engineering and Applied Chemistry, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada. The homeostatic adult bone marrow (BM) is a complex tissue wherein physical and biochemical interactions serve to maintain a balance between the hematopoietic and nonhematopoietic compartments. To focus on soluble factor interactions occurring between mesenchymal and hematopoietic cells, a serum-free adhesion-independent culture system was developed that allows manipulation of the growth of both mesenchymal and hematopoietic human BM-derived progenitors and the balance between these compartments. Factorial experiments demonstrated a role for stem cell factor (SCF) and interleukin 3 (IL-3) in the concomitant growth of hematopoietic (CD45+) and nonhematopoietic (CD45–) cells, as well as their derivatives. Kinetic tracking of IL-3 receptor (CD123) and SCF receptor (CD117) expression on a sorted CD45– cell population revealed the emergence of CD45–CD123+ cells capable of osteogenesis. Of the total fibroblast colony-forming units (CFU-Fs) and osteoblast colony-forming units (CFU-O), approximately 24% of CFU-Fs and about 22% of CFU-Os were recovered from this population. Cell-sorting experiments demonstrated that the CD45+ cell population secreted soluble factors that positively affect the survival and proliferation of CFU-Fs and CFU-Os generated from the CD45– cells. Together, our results provide insight into the intercellular cytokine network between hematopoietic and mesenchymal cells and provide a strategy to mutually culture both mesenchymal and hematopoietic cells in a defined scalable bioprocess. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Barese, N. Pech, S. Dirscherl, J. L. Meyers, A. L. Sinn, M. C. Yoder, W. S. Goebel, and M. C. Dinauer Granulocyte Colony-Stimulating Factor Prior to Nonmyeloablative Irradiation Decreases Murine Host Hematopoietic Stem Cell Function and Increases Engraftment of Donor Marrow Cells Stem Cells, June 1, 2007; 25(6): 1578 - 1585. [Abstract] [Full Text] [PDF] X. Chen, H. Xu, C. Wan, M. McCaigue, and G. Li Bioreactor Expansion of Human Adult Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells, September 1, 2006; 24(9): 2052 - 2059. [Abstract] [Full Text] [PDF]

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