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Blood, 1 January 2006, Vol. 107, No. 1, pp. 205-212. Prepublished online as a Blood First Edition Paper on September 6, 2005; DOI 10.1182/blood-2005-05-2155. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-05-2155v1 107/1/205    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yong, A. S. M. Articles by Melo, J. V. Search for Related Content PubMed PubMed Citation Articles by Yong, A. S. M. Articles by Melo, J. V. Related Collections Neoplasia Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Molecular profiling of CD34+ cells identifies low expression of CD7, along with high expression of proteinase 3 or elastase, as predictors of longer survival in patients with CML Agnes S. M. Yong, Richard M. Szydlo, John M. Goldman, Jane F. Apperley, and Junia V. Melo From the Department of Haematology, Imperial College London, Hammersmith Hospital, London, United Kingdom. Although most patients with chronic myeloid leukemia (CML) have the same initial molecular abnormality, the BCR-ABL fusion gene, the duration of chronic phase (CP) varies widely. To identify the possible molecular basis of this heterogeneity, we studied CD34+ cells collected at diagnosis from 68 patients with CML-CP. By using oligonucleotide microarray screening, we performed gene-expression profiling on 2 subsets of patients, one comprising patients with an "aggressive disease" who developed blastic transformation (BT) within 3 years of diagnosis (n = 10) and, at the other extreme, patients with an "indolent disease" whose BT occurred 7 or more years from diagnosis (n = 9). This screening revealed 20 genes differentially expressed in patients with aggressive and indolent disease, which were validated by quantitative reverse transcriptase/polymerase chain reaction (Q-RT/PCR). A multivariate Cox regression model identified the combination of low CD7 expression with high expression of proteinase 3 or elastase as associated with longer survival in the complete cohort of 68 patients. This differential pattern of gene expression probably reflects the intrinsic heterogeneity of the disease; if so, assessing expression levels of selected genes at diagnosis may be valuable in predicting duration of survival in patients treated with imatinib and the newer tyrosine kinase inhibitors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: V. G. Oehler, K. Y. Yeung, Y. E. Choi, R. E. Bumgarner, A. E. Raftery, and J. P. Radich The derivation of diagnostic markers of chronic myeloid leukemia progression from microarray data Blood, October 8, 2009; 114(15): 3292 - 3298. [Abstract] [Full Text] [PDF] J. S. Khorashad, S. Wagner, L. Greener, D. Marin, A. Reid, D. Milojkovic, H. Patel, S. Willimott, K. Rezvani, G. Gerrard, et al. 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