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Blood, 15 May 2006, Vol. 107, No. 10, pp. 3865-3867. Prepublished online as a Blood First Edition Paper on January 26, 2006; DOI 10.1182/blood-2005-10-4108. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-10-4108v1 107/10/3865    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Seggewiss, R. Articles by Dunbar, C. E. Search for Related Content PubMed PubMed Citation Articles by Seggewiss, R. Articles by Dunbar, C. E. Related Collections Hematopoiesis and Stem Cells Neoplasia Stem Cells in Hematology Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  GENE THERAPY Brief report Acute myeloid leukemia is associated with retroviral gene transfer to hematopoietic progenitor cells in a rhesus macaque Ruth Seggewiss, Stefania Pittaluga, Rima L. Adler, F. Javier Guenaga, Cole Ferguson, Ingo H. Pilz, Byoung Ryu, Brian P. Sorrentino, W. Scott Young, III, Robert E. Donahue, Christof von Kalle, Arthur W. Nienhuis, and Cynthia E. Dunbar From the Hematology Branch, National Heart, Lung, and Blood Institute (NHLBI), the Hematopathology Section, National Cancer Institute (NCI), and the Section on Neural Gene Expression, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services (NIH/DHHS), Bethesda, MD; the Division of Experimental Hematology, Hematology-Oncology, St Jude's Children's Research Hospital, Memphis, TN; the University of Freiburg, Department of Medicine I, Freiburg, Germany; and the Children's Hospital Research Foundation, Molecular and Gene Therapy Program, Cincinnati, OH. We report, for the first time, a replication-defective retroviral vector–associated neoplasia in a nonhuman primate. Five years after transplantation with CD34+ cells transduced with a retroviral vector expressing enhanced green fluorescent protein (eGFP) and a drug-resistant variant of the dihydrofolate reductase gene (L22Y), a rhesus macaque developed a fatal myeloid sarcoma, a type of acute myeloid leukemia. Tumor cells contained 2 clonal vector insertions. One insertion was found in BCL2-A1, an antiapoptotic gene. This event suggests that currently available retroviral vectors may have long-term side effects, particularly in hematopoietic stem and progenitor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. J. POSEKANY, J. E. WILEY, and G. A. GAGNON A Novel Method to Display [gal {alpha}1, 3 gal] Antigens on Human Leukemic Cells for Preparation of Anti-leukemia Vaccines Anticancer Res, June 1, 2009; 29(6): 2387 - 2392. [Abstract] [Full Text] [PDF] Y.-J. Kim, Y.-S. Kim, A. Larochelle, G. Renaud, T. G. 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