|
|
Blood, 15 June 2006, Vol. 107, No. 12, pp. 4746-4753.
Prepublished online as a Blood First Edition Paper on February 21, 2006; DOI 10.1182/blood-2005-11-4674.
 Previous Article | Table of Contents | Next Article 
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Cellular localization and trafficking of tissue factor
Samir K. Mandal,
Usha R. Pendurthi, and
L. Vijaya Mohan Rao
From the Biomedical Research Division, The University of Texas Health Center at Tyler, TX.
Tissue factor (TF) is the cellular receptor for clotting factor VIIa (FVIIa). The formation of TF-FVIIa complexes on cell surfaces triggers the activation of coagulation cascade and cell signaling. In the present study, we characterized the subcellular distribution of TF and its transport in fibroblasts by dual immunofluorescence confocal microscopy and biochemical methods. Our data show that a majority of TF resides in various intracellular compartments, predominantly in the Golgi. Tissue factor at the cell surface is localized in cholesterol-rich lipid rafts and extensively colocalized with caveolin-1. FVIIa binding to TF induces the internalization of TF. Of interest, we found that TF-FVIIa complex formation at the cell surface leads to TF mobilization from the Golgi with a resultant increase in TF expression at the cell surface. This process is dependent on FVIIa protease activity. Overall, the present data suggest a novel mechanism for TF expression at the cell surface by FVIIa. This mechanism could play an important role in hemostasis in response to vascular injury by increasing TF activity where and when it is needed.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. T.B. Crawley and D. A. Lane
The Haemostatic Role of Tissue Factor Pathway Inhibitor
Arterioscler. Thromb. Vasc. Biol.,
February 1, 2008;
28(2):
233 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. H. Versteeg, F. Schaffner, M. Kerver, H. H. Petersen, J. Ahamed, B. Felding-Habermann, Y. Takada, B. M. Mueller, and W. Ruf
Inhibition of tissue factor signaling suppresses tumor growth
Blood,
January 1, 2008;
111(1):
190 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. R. Pendurthi, S. Ghosh, S. K. Mandal, and L. V. M. Rao
Tissue factor activation: is disulfide bond switching a regulatory mechanism?
Blood,
December 1, 2007;
110(12):
3900 - 3908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Mandal, U. R. Pendurthi, and L. V. M. Rao
Tissue factor trafficking in fibroblasts: involvement of protease-activated receptor-mediated cell signaling
Blood,
July 1, 2007;
110(1):
161 - 170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Satta, S. Dunoyer-Geindre, G. Reber, R. J. Fish, F. Boehlen, E. K. O. Kruithof, and P. de Moerloose
The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies
Blood,
February 15, 2007;
109(4):
1507 - 1514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Mandal, U. R. Pendurthi, and L. V. M. Rao
Tissue Factor Internalization and Trafficking in Fibroblasts: Involvement of Protease Activated Receptors-Mediated Cell Signaling.
Blood (ASH Annual Meeting Abstracts),
November 16, 2006;
108(11):
541 - 541.
[Abstract]
[PDF]
|
|
|
|
|
