|
|
Blood, 15 June 2006, Vol. 107, No. 12, pp. 4798-4806.
Prepublished online as a Blood First Edition Paper on February 23, 2006; DOI 10.1182/blood-2005-09-3581.
 Previous Article | Table of Contents | Next Article 
IMMUNOBIOLOGY
Use of CD44 by CD4+ Th1 and Th2 lymphocytes to roll and adhere
Claudine S. Bonder,
Stephen R. Clark,
M. Ursula Norman,
Pauline Johnson, and
Paul Kubes
From the Immunology Research Group, Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, AB, Canada; Vascular Biology Laboratory, Department of Human Immunology, Hanson Institute and Institute for Medical and Veterinary Science, Adelaide, Australia; and Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada.
Localization of circulating lymphocytes to a site of inflammation is paramount for the development and maintenance of an immune response. In vitro studies using cell lines have previously demonstrated that rolling and adhesion of lymphocytes on endothelium requires CD44 interactions with hyaluronan (HA). To date, whether CD44 has a role in mediating CD4+-polarized T-helper 1 (Th1) and Th2 lymphocyte interactions with the endothelium in vivo is yet to be determined. In this study we used intravital microscopy to demonstrate that both Th1 and Th2 lymphocytes use CD44 to roll and adhere to tumor necrosis factor- (TNF)–activated microvasculature. Furthermore, chimeric studies imply that CD44 expression by both the endothelium and lymphocytes is essential for these interactions to occur. HA was also necessary for T cell–endothelial cell interactions in vivo and Th1 and Th2 cells rolled on immobilized HA in vitro via CD44. In vitro, both Th1 and Th2 lymphocytes have increased expression of CD44 and greater binding of fluorescent HA than naive cells. The interactions of Th1 and Th2 cells were entirely dependent upon both P-selectin and CD44 in vivo, but did not appear to be counter ligands in vitro. Taken together, these results suggest that CD44 and HA are key to both Th1 and Th2 lymphocyte interactions with the TNF-activated endothelium and raises the possibility of cooperativity between the P-selectin/PSGL-1 and HA/CD44 pathways for Th1 and Th2 rolling in vivo.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. McDonald, E. F. McAvoy, F. Lam, V. Gill, C. de la Motte, R. C. Savani, and P. Kubes
Interaction of CD44 and hyaluronan is the dominant mechanism for neutrophil sequestration in inflamed liver sinusoids
J. Exp. Med.,
April 14, 2008;
205(4):
915 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Jokela, M. Jauhiainen, S. Auriola, M. Kauhanen, R. Tiihonen, M. I. Tammi, and R. H. Tammi
Mannose Inhibits Hyaluronan Synthesis by Down-regulation of the Cellular Pool of UDP-N-acetylhexosamines
J. Biol. Chem.,
March 21, 2008;
283(12):
7666 - 7673.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Katoh, N. Ishii, A. Nobumoto, K. Takeshita, S.-Y. Dai, R. Shinonaga, T. Niki, N. Nishi, A. Tominaga, A. Yamauchi, et al.
Galectin-9 Inhibits CD44-Hyaluronan Interaction and Suppresses a Murine Model of Allergic Asthma
Am. J. Respir. Crit. Care Med.,
July 1, 2007;
176(1):
27 - 35.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
